外源性mTκα基因抑制肝癌细胞HepG2的生长

摘要/Abstract

摘要： 目的观察核转录因子的抑制基因mTκα在肝癌细胞株HepG2的表达及对该细胞株生长的影响。方法将由293包装细胞产生的含mTκα的Ad腺病毒上清(Ad-mIκBα)和含包装载体的腺病毒上清(Adv)感染HepG2细胞,观察感染后HepG2细胞的绿色荧光蛋白(GFP)表达和mIκBα蛋白表达,并观察细胞的平板集落形成数、软琼脂集落形成能力、细胞生长曲线、裸鼠成瘤实验。结果肝癌细胞感染腺病毒后GFP表达阳性,感染含mIκBαAd腺病毒的肝癌细胞有mIκBα蛋白表达;HepG2/Ad-mIκBα细胞形成的集落数明显比HepG2/Adv和HepG2细胞形成的集落数少(P<0.05);HepG2细胞、HepG2/Adv细胞在软琼脂内存活,并形成桑椹样细胞集落,而HepG2/Ad-mIκBα细胞在软琼脂上无生长;细胞生长曲线显示HepG2/Ad-mIκBα细胞生长速度较HepG2/Adv、HepG2亲本细胞有所减慢,但饱和密度无显著性差异。裸鼠接种4周后HepG2/Ad-mIκBα细胞成瘤率为80%,HepG2/Adv细胞成瘤率为100%,肿瘤形成率无显著性差异,HepG2/Ad-mIκBα细胞形成的肿瘤的体积比HepG2/Adv细胞形成的肿瘤的体积明显减小(P<0.05)。结论mTκα基因可以抑制HepG2肝癌细胞的生长。

Abstract: Objective To observe the expression of mTκα,the inhibitor of NFκB,in HepG2 heptocellular carcinoma cells and the inhibitory effect of mTκα;α on HepG2 cell growth.Methods The adenovirus containing mTκα or the packaging vector were harvested from 293 packaging cells to infect HepG2 cells,in which the expression of green fluorescence protein (GFP) and mIκBα protein were detected. The plating efficiency and colony-forming ability in soft agar were evaluated,cell growth curve was generated and the tumor growth observed in nude mice. Results HepG2 cells infected by the packaged adenovirus (HepG2/Adv cells) had positive GFP expression,and those infected by the adenovirus containing mIκBα (HepG2/Ad-mIκBα cells) showed mIκBα protein expression,as demonstrated by Western blot analysis. HepG2/Adv cells exhibited markedly lowered colony-forming ability as compared with that of the HepG2/Adv and HepG2 cells,and the latter two cells,but not the former cells,could survive in soft agar and form colonies in the shape mulberries. Cell growth curve showed that the Hep G2/Ad-mIκBα cells had also significantly reduced growth rate,but their saturation density in culture was comparable with the other two cells. Four weeks after inoculation in nude mice,HepG2/Ad-mIκBα and HepG2/Adv cells showed no significant difference in tumorigenicity (80%vs100%,respectively),but the tumor volume generated by the former cells was significantly smaller (P<0.05).Conclusion mTκα gene can inhibit the growth of HepG2 heptocellular carcinoma cells.

中图分类号:

R392.
R735.7

金吴东¹, 陈龙华¹, 穆峰². 外源性mTκα基因抑制肝癌细胞HepG2的生长[J]. 南方医科大学学报, 2004, 24(10): 1133-1136.

JIN Wu-dong¹, CHEN Long-hua¹, MU Feng². Exogenous mTκα gene inhibits the growth of HepG2 heptocellular carcinoma cells[J]. Journal of Southern Medical University, 2004, 24(10): 1133-1136.

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