大鼠骨髓源干细胞在损伤脑组织中的分化趋势

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (10): 1102-1106.

大鼠骨髓源干细胞在损伤脑组织中的分化趋势

邹西峰¹, 张海燕², 赵春礼², 李铁林¹, 徐群渊²

1. 南方医科大学珠江医院神经外科, 广东, 广州, 510282;
2. 首都医科大学北京神经科学研究所, 北京市神经再生修复研究重点实验室, 北京, 100054

出版日期:2004-10-20 发布日期:2004-10-20
基金资助:
收稿日期:2004-7-2。
基金项目:国家重点基础研究规划(G1999054008)
作者简介:邹西峰(1966- ),男,博士,副主任医师,现工作单位为华北煤炭医学院附属医院神经外科,电话:020-61643263
通讯作者:徐群渊,电话:010-63051103

Differentiation of bone marrow-derived stem cells in injured rat brain tissue

ZOU Xi-feng¹, ZHANG Hai-yan², ZHAO Chun-li², LI Tie-lin¹, XU Qun-yuan²

1. 南方医科大学珠江医院神经外科, 广东, 广州, 510282;
2. 首都医科大学北京神经科学研究所, 北京市神经再生修复研究重点实验室, 北京, 100054

Online:2004-10-20 Published:2004-10-20

摘要/Abstract

摘要： 目的探讨迁移至损伤脑组织的骨髓源干细胞(BMDSCs)的分化趋势。方法将雌性SD大鼠脑损伤模型在制作好24h后经尾静脉植入GFP标记的雄性SD大鼠的BMDSCs。在植入后2周、4周和8周分批取脑,分别用CD11b和GFAP荧光免疫组织化学方法检测GFP阳性细胞的性质。结果在移植GFP标记的BMDSCs后1周,植入的GFP阳性BMDSCs占外周血白细胞总数的3.53%;移植BMDSCs后4周和8周的脑损伤病灶周围有集中分布的GFP阳性细胞,分别表达小胶质细胞的细胞表面标记CD11b和胶质纤维酸性蛋白GFAP。结论 GFP标记的BMDSCs能迁移至脑损伤病灶周围并有向小胶质细胞和星形胶质细胞分化的趋势。

Abstract: Objective To investigate the differentiation of bone marrow-derived stem cells (BMDSCs) migrated from blood circulation and resided in the injured brain tissue.Methods Brain injury model was established by iridectomy in the right cerebral cortex of female SD rats. Twenty-four hours after brain injury,the female rats received the implantation of green fluorescence protein (GFP)-labeled BMDSCs from male SD rats and were sacrificed at 2,4 and 8 weeks after the implantation. Fluorescent immunohistochemistry for CD11b and glial fibrillary acidic protein (GFAP) on the brain sections was used to detect the GFP-positive cells. Results One week after the transplantation of the GFP-labeled BMDSCs,3.53% of the peripheral blood white cells were GFP-positive; at 4 weeks and 8 weeks,a significant number of GFP-positive cells were found at the injury sites,some of which expressed CD11b and others expressed GFAP.Conclusion GFP-labeled BMDSCs can migrate to the injured brain tissue and differentiate into cells that express microglia- and astrocytes-specific antigens.

中图分类号:

Q254
Q189

邹西峰¹, 张海燕², 赵春礼², 李铁林¹, 徐群渊². 大鼠骨髓源干细胞在损伤脑组织中的分化趋势[J]. 南方医科大学学报, 2004, 24(10): 1102-1106.

ZOU Xi-feng¹, ZHANG Hai-yan², ZHAO Chun-li², LI Tie-lin¹, XU Qun-yuan². Differentiation of bone marrow-derived stem cells in injured rat brain tissue[J]. Journal of Southern Medical University, 2004, 24(10): 1102-1106.

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