DNA聚丙烯酰胺凝胶电泳银染方法的选择

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (09): 1072-1074.

DNA聚丙烯酰胺凝胶电泳银染方法的选择

李西平, 钱新华, 姚英民, 刘志杰, 刘阳

第一军医大学南方医院儿科, 广东, 广州, 510515

出版日期:2004-09-20 发布日期:2004-09-20
基金资助:
收稿日期:2003-5-12。
基金项目:国家自然科学基金(39770784)
作者简介:李西平(1969-),女,第一军医大学在读博士研究生,电话:020-61641929

Comparison of several silver staining methods for DNA polyacrylamide gel electrophoresis

LI Xi-ping, QIAN Xin-hua, YAO Ying-min, LIU Zhi-jie, LIU Yang

第一军医大学南方医院儿科, 广东, 广州, 510515

Online:2004-09-20 Published:2004-09-20

摘要/Abstract

摘要： 目的比较4种聚丙烯酰胺凝胶银染方法,为初次使用者选择方法时提供参考。方法用24 mer的引物作为上样样本,分别采用氨水法、低浓度硝酸银法、0.1%及0.2%硝酸银法染色。后2种配合不同的显色剂显色。结果前2种未显色,0.1%及0.2%硝酸银可显色。结论 0.2%的硝酸银与氢氧化钠显色剂相结合具有最高的灵敏度;0.2%的硝酸银与碳酸钠显色剂相结合具有最佳的显像效果。

Abstract: Objective To compare 4 silver staining methods for DNA detection in polyacrylamide gel electrophoresis. Methods After the electrophoresis was completed, the gels were stained separately by four different methods, namely using ammonia-silver-citric acid, low-concentration silver nitrate, 0.1% and 0.2% silver nitrate. Results DNA was detected only by staining with 0.1% and 0.2% silver nitrate. Conclusion 0.2% silver nitrate used along with sodium hydroxide shows the highest sensitivity in DNA detection, while its use with sodium carbonate produces the best quality of the image.

中图分类号:

Q329.34

李西平, 钱新华, 姚英民, 刘志杰, 刘阳. DNA聚丙烯酰胺凝胶电泳银染方法的选择[J]. 南方医科大学学报, 2004, 24(09): 1072-1074.

LI Xi-ping, QIAN Xin-hua, YAO Ying-min, LIU Zhi-jie, LIU Yang. Comparison of several silver staining methods for DNA polyacrylamide gel electrophoresis[J]. Journal of Southern Medical University, 2004, 24(09): 1072-1074.

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