Abstract: Objective To investigate the clinical effect of continuous suture technique for closure of surgically created arteriovenous fistulas at different sites. Method A total of 160 patients with chronic renal failure underwent surgery to create arteriovenous fistulas at different sites, which were closed with continuous suture technique. Results The time for vessel anastomosis was reduced with this suture technique, and the success rate of anastomosis at one time and the overall operative success rate were improved, with also reduction of complications to achieve satisfactory clinical results. Conclusion Continuous suture technique well suits the demand in anastomosis of arteriovenous fistula at different sites, especially in cases with poor vascular condition.