南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (09): 1029-1032,1036.

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珍珠层/聚乳酸重组人工骨修复动物骨干缺损的实验研究

刘金标1, 廉维2, 陈建庭3, 金大地3, 权毅1, 潘显明1   

  1. 1. 成都军区总医院, 骨科, 四川, 成都, 610083;
    2. 成都军区总医院, 输血科, 四川, 成都, 610083;
    3. 第一军医大学南方医院脊柱骨病科、全军脊柱外科中心, 广东, 广州, 510515
  • 出版日期:2004-09-20 发布日期:2004-09-20
  • 基金资助:
    收稿日期:2003-8-20。
    基金项目:广东省卫生厅基金(A1998333)
    作者简介:刘金标(1970-),男,2001年毕业于第一军医大学,硕士,主治医师,电话:028-86570365

Diaphyseal defect repair with nacre/polylactic acid composite artificial bone in rabbits

LIU Jin-biao1, LIAN Wei2, CHEN Jian-ting3, JIN Da-di3, QUAN Yi1, PAN Xian-ming1   

  1. 1. 成都军区总医院, 骨科, 四川, 成都, 610083;
    2. 成都军区总医院, 输血科, 四川, 成都, 610083;
    3. 第一军医大学南方医院脊柱骨病科、全军脊柱外科中心, 广东, 广州, 510515
  • Online:2004-09-20 Published:2004-09-20

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摘要： 目的 探讨珍珠层/聚乳酸重组人工骨(NPCB)在体内的生物相容性、降解情况及成骨特点,评价其性能,为进一步的应用提供实验依据。方法 将NPCB随机植入成年新西兰白兔1.5 cm的桡骨缺损内,并设立不植入任何材料的空白对照,观察NPCB植入后动物的局部反应,检测动物术后1周、4周的血钙值,并与术前1天比较;于术后6、8、12、16周取材,作X线、骨矿含量、大体标本及组织形态学观察,分析不同时期组织反应、骨缺损修复及材料降解情况。结果 NPCB植入后无明显的局部不良反应,术后1周、4周血钙值与术前1天相比无明显差异。植入NPCB的骨缺损内骨矿含量在6~12周时升高幅度明显高于空白对照组,但12周后植入NPCB的骨缺损内骨矿含量出现下降。X线检查、大体标本及组织形态学观察显示NPCB的成骨方式主要是骨传导成骨,至术后16周时骨缺损基本修复,NPCB与宿主骨结合紧密,而空白对照骨缺损断端仅有少量骨修复,形成骨不连。NPCB植入后即开始其降解过程,12周以后材料周围出现较多吞噬有材料颗粒的巨噬细胞和多核巨细胞,16周时仍有部分材料未降解吸收。结论 NPCB具备良好的生物相容性和骨传导能力,可以在体内逐渐发生生物降解。

Abstract: Objective To evaluate the biocompatibility, degradation and bone formation activity of a new bone substitute for bone grafting, nacre/polylactic acid composite artificial bone (NPCB). Methods Radial bone defects 1.5 cm in length were induced in 32 New Zealand rabbits and immediately filled with NPCB or nothing. The animals’ local and whole body responses to the implants were observed after the operation, and the serum calcium levels were detected 1 week and 4 weeks postoperatively. Tissue response, new bone formation in the defects and degradation of the implants were evaluated by X-ray, and examination of the bone mineral content (BMC) in the defects and histomorphological analysis were performed after the rabbits were sacrificed. Results All the rabbits survived the operation and the incisions healed smoothly. No significant difference was noted in the serum calcium level between 1 day before operation, 1 week and 4 weeks postoperation. BMC in the defects of the rabbits with NPCB grafts increased more rapidly than that in the control group 6-12 weeks after the operation and began to decrease after 12 weeks. Gross, X-ray and histological observations revealed that NPCB possessed osteoconductive activity and new bone ingrowth in the implants was found during the whole experiment. At 16 weeks, the defects grafts were almost completely repaired with NPCB while the defects in the control group remained nonunion. Biodegradation of the implants was observed early at 6 weeks, with numerous macrophage and multinuclear giant cells containing phagocytosed NPCB particles around the implants at 12 weeks, but NPCB remnants were still visible at 16 weeks. Conclusion As a bone substitute, NPCB possesses good biocompatibility, biodegradability and osteoconductive activity.

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