基因芯片筛选大肠侧向发育型肿瘤相关基因的初步研究

摘要/Abstract

摘要： 目的应用基因芯片初步筛选大肠侧向发育型肿瘤(LST)相关基因,为进一步研究大肠癌的发病机制提供新的思路和线索。方法抽提大肠侧向发育型肿瘤癌细胞株、SW480细胞株、LoVo细胞株的总RNA并纯化mRNA;将18 816种人类基因及LST片段PCR产物用Biorobotics公司的BG600点样仪点样于纤维膜上,制成基因芯片。将3个样品的mRNA分别逆转录合成探针并³³P标记,同芯片杂交。严格洗片后Fuji film公司FLA 3000扫描仪扫描芯片信号图象,Array Gauge1.0软件分析比较3种细胞株中差异表达基因。结果和结论 在18 816种人类基因中存在着一系列LST同普通大肠癌细胞株之间的差异表达基因。在本研究中共有差异表达基因97个,其中共上调58个,共下调39个。表明其可能存在不同的肿瘤发生机制,故进一步研究其相关基因,在分子水平上阐明其发生机制及与大肠癌的关系具有重要意义。

Abstract: Objective To screen differentially expressed genes between laterally spreading tumor (LST) cell line and common colon carcinoma cell lines, and identify new targets and strategies for exploring the pathogenesis of colorectal tumor. Methods The total RNA was extracted from the LST, SW480 and LoVo cells, from which purified mRNAs were obtained. The PCR products of 18 816 genes were blotted onto a fibrous membrane to generate the microarray. The mRNAs from the 3 cell lines were reversely transcribed into cDNA probes and labeled with ³³P before hybridization with the cDNA microarray. After thorough washing, the cDNA microarray was scanned and the 3 samples compared. Results and Conclusions A series of differentially expressed genes were found between the 3 samples, and 58 up-regulated and 39 down-regulated genes were identified among the 97 differentially expressed genes, which suggest different pathogeneses of the laterally spreading tumor. Further analysis of the obtained genes can be helpful in understanding the molecular mechanism of colorectal tumors.

中图分类号:

R735.34

王新颖, 姜泊, 赖卓胜, 马文敏, 耿焱. 基因芯片筛选大肠侧向发育型肿瘤相关基因的初步研究[J]. 南方医科大学学报, 2004, 24(09): 1023-1025.

WANG Xin-ying, JIANG Bo, LAI Zhuo-sheng, MA Wen-min, GENG Yan. Screening of differentially expressed genes related to laterally spreading tumor by cDNA microarray[J]. Journal of Southern Medical University, 2004, 24(09): 1023-1025.

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