肝缺血再灌注损伤早期对其他脏器的影响

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (09): 1019-1022.

肝缺血再灌注损伤早期对其他脏器的影响

杨进城, 季锡清, 李朝龙, 林建华, 刘兴国

第一军医大学南方医院肝胆血管外科, 广东, 广州, 510515

出版日期:2004-09-20 发布日期:2004-09-20
基金资助:
收稿日期:2003-11-29。
基金项目:广东省计划攻关项目(02B30204)
作者简介:杨进城(1975-),男,硕士,电话:020-33161962,E-mail:ycheng75@sohu.com

Impact of early-stage hepatic ischemia-reperfusion injury on other organs of rats

YANG Jin-cheng, JI Xi-qing, LI Chao-long, LIN Jian-hua, LIU Xing-guo

第一军医大学南方医院肝胆血管外科, 广东, 广州, 510515

Online:2004-09-20 Published:2004-09-20

摘要/Abstract

摘要： 目的了解肝缺血再灌注损伤对其他重要脏器功能的影响。方法雄性SD大鼠25只,按阻断血流前后不同时间点均分为5组,即肝血流阻断前、阻断30 min末、再灌注后30 min、再灌注后2 h及再灌注后6 h。各组分别活杀5只大鼠取血检测血生化指标,同时取心肌组织测ATPase活性;在缺血前及再灌注后6 h取肾、肺、心、胰组织匀浆测丙二醛、超氧化物歧化酶。结果再灌注后早期血浆丙氨酸氨基转移酶、天冬氨酸氨基转移酶、血尿素氮、淀粉酶、心肌型肌酸激酶同功酶及血浆丙二醛呈上升趋势,血浆超氧化物歧化酶及组织ATP酶活性在再灌注后呈下降趋势。再灌注后6 h,各组织丙二醛含量较缺血前明显升高,而组织超氧化物歧化酶较缺血前明显下降。结论肝缺血再灌注早期可引起机体其他多脏器的损伤。

Abstract: Objective To investigate the influence of hepatic ischemia-reperfusion on other organs of rats during the early stage of injury. Methods Twenty-five healthy male SD rats were randomized equally into 5 groups according to the different time points in the course of hepatic vascular occlusion and reperfusion, namely pre-occlusion, the end of occlusion, and after 0.5, 2.0 and 6.0 h reperfusion, respectively. In each group the rats were killed to obtain samples at the specified time points. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) in the lung, kidney, pancreas and heart were measured in pre-occlusion and 6-hour reperfusion groups. The triphosphate (ATPase) activity in heart tissues, the contents of MDA and the activity of SOD in blood and the blood biochemistry were observed in different groups. Results The activity of SOD and ATPase were decreased (P<0.05) after the ischemia-reperfusion as compared with those before ischemia, but the contents of MDA, ALT, AST BUN, AMY and CK-MB increased significantly (P<0.05). Conclusion Liver ischemia-reperfusion can induce injury to other organs at the early stage of reperfusion.

中图分类号:

R657.31

杨进城, 季锡清, 李朝龙, 林建华, 刘兴国. 肝缺血再灌注损伤早期对其他脏器的影响[J]. 南方医科大学学报, 2004, 24(09): 1019-1022.

YANG Jin-cheng, JI Xi-qing, LI Chao-long, LIN Jian-hua, LIU Xing-guo. Impact of early-stage hepatic ischemia-reperfusion injury on other organs of rats[J]. Journal of Southern Medical University, 2004, 24(09): 1019-1022.

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