两株诺瓦克样病毒新亚型的鉴定

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (09): 1016-1018,1022.

两株诺瓦克样病毒新亚型的鉴定

戴迎春¹, 聂军¹, 刘翼¹, 李志峰¹, 白杨², 李建栋¹, 俞守义¹, 江熙²

1. 第一军医大学流行病学教研室, 广东广州, 510515;
2. 辛辛那提儿科研究中心, 辛辛那提, 美国

出版日期:2004-09-20 发布日期:2004-09-20
基金资助:
收稿日期:2004-4-22。
基金项目:广东省医学科研基金(A2003420)
作者简介:戴迎春(1979-),女,第一军医大学在读硕士研究生,电话:020-61648311,E-mail:yingchun78@hotmail.com

Identification of two new strains of norovirus in Guangzhou

DAI Ying-chun¹, NIE Jun¹, LIU Yi¹, LI Zhi-feng¹, BAI Yang², LI Jian-dong¹, YU Shou-yi¹, JIANG Xi²

1. 第一军医大学流行病学教研室, 广东广州, 510515;
2. 辛辛那提儿科研究中心, 辛辛那提, 美国

Online:2004-09-20 Published:2004-09-20

摘要/Abstract

摘要： 目的对在广州检出的人类杯状病毒(HuCVs)毒株进行分子生物学鉴定,以确定其是否为新亚型。方法选择检出的HuCVs进行克隆、转化和序列分析,使用PHYLIP进行核酸序列同源性比较,经Treeview1.5绘制进化树。结果 206份病毒性腹泻标本检出HuCVs18份,检出率为8.74%(18/206),经序列分析证实均为NVs GⅡ毒株。任选其中6株进行PCR产物的克隆和测序,与GenBank中的参考株比较并绘制遗传进化树,其中HuCV/NVGⅡ003/2003/CHN(32282)、HuCV/NVGⅡ004/2003/CHN(32283)与基因文库中病毒的最高同源性分别为83%和87%。结论广州存在其他国家或地区没有报道过的诺瓦克样病毒新亚型毒株。

Abstract: Objective To identify the molecular biological characteristics of human caliciviruses (HuCVs) and new strains of norovirus (NV) detected in Guangdong Province. Methods HuCVs were detected from the stool specimens by reverse transcription-PCR (RT-PCR), and the PCR products were cloned, transformed, and blasted with Genbank.The nucleotide sequence homology was also evaluated with PHYLIP package, and the phylogenetic tree was generated by Treeview. Results HuCVs were detected from 18 out of 206 stool specimens from children with viral diarrhea (8.74%), which were all confirmed subsequently as NVs. Two strains HuCV/NVGⅡ 003/2003/CHN(32282) and HuCV/NVGⅡ 004/2003/CHN(32283) from the selected 6 NVs strain were identified as new NV strains. Conclusion New NV strains in consistency with the blood-type distribution of Chinese are present in Guangzhou, which have not been reported in other countries.

中图分类号:

R373

戴迎春¹, 聂军¹, 刘翼¹, 李志峰¹, 白杨², 李建栋¹, 俞守义¹, 江熙². 两株诺瓦克样病毒新亚型的鉴定[J]. 南方医科大学学报, 2004, 24(09): 1016-1018,1022.

DAI Ying-chun¹, NIE Jun¹, LIU Yi¹, LI Zhi-feng¹, BAI Yang², LI Jian-dong¹, YU Shou-yi¹, JIANG Xi². Identification of two new strains of norovirus in Guangzhou[J]. Journal of Southern Medical University, 2004, 24(09): 1016-1018,1022.

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