转染人pIgR的小鼠鼻咽上皮细胞在EBV感染前后基因表达谱差异

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (09): 1001-1005.

转染人pIgR的小鼠鼻咽上皮细胞在EBV感染前后基因表达谱差异

王爽, 吕丽春, 李虹, 江培洲, 姚开泰

第一军医大学病理学教研室, 广东, 广州, 510515

出版日期:2004-09-20 发布日期:2004-09-20
基金资助:
收稿日期:2004-2-2。
基金项目:广东省“十五”社会发展攻关专项基金(2001A1080201);全军医药卫生科研基金(01Z048)
作者简介:王爽(1977-),女,第一军医大学病理学专业在读研究生,电话:020-61640114-89100,E-mail:wang3660@fimmu.com

Difference in gene expression profile of human polymeric immunoglobulin receptor-transfected mouse nasopharyngeal epithelial cells before and after EBV infection

WANG Shuang, LÜ Li-chun, LI Hong, JIANG Pei-zhou, YAO Kai-tai

第一军医大学病理学教研室, 广东, 广州, 510515

Online:2004-09-20 Published:2004-09-20

摘要/Abstract

摘要： 目的应用cDNA阵列技术研究转染人多聚免疫球蛋白受体(hpIgR)的二亚硝基哌嗪(n, n’-dinitrosoperazine, DNP)转化的小鼠鼻咽上皮(TMNE)细胞系在EBV感染前后基因表达谱差异,探索EBV在鼻咽癌发病过程中的作用及鼻咽癌的发病机制。方法提取转染hpIgR 的TMNE细胞在EBV感染前后的总RNA,逆转录成α-³²P-dATP标记的cDNA探针,与AtlasTM mouse cancer array 1.2 阵列膜进行杂交。利用AtlasImage^TM 软件分析基因表达差异。结果共有25个基因表达水平发生了改变,有23个基因表达上调,2个基因表达下调。结论 EBV感染可使转染细胞的多个基因表达发生改变,这些基因可能与鼻咽癌的发生、发展有关。

Abstract: Objective To study the gene expression profile of human polymeric immunoglobulin receptor gene (hpIgR)-transfected mouse nasopharyngeal epithelial cells transformed with n, n’-dinitrosoperazine (TMNE) before and after EBV infection using cDNA array and investigate the role of Epstein-Barr virus (EBV) infection in the tumorigenesis of nasopharyngeal carcinoma (NPC). Methods The total RNAs of hpIgR-transfected TMNE cells before and after EBV infection were extracted, reversely transcribed, and labeled with α-³²P-dATP. The cDNA probes were hybridized to the AtlasTM mouse cancer array 1.2, and the signals analyzed by AtlasImage^TM software. Results Twenty-five genes differentially expressed in cells before and after EBV infection, including 23 up-regulated genes and 2 down-regulated genes. Conclusions The gene expression profile of hpIgR-transfected TMNE cells may change after EBV infection, suggesting that these genes are probably involved in the tumorigenesis and progression of NPC.

中图分类号:

R730.231

王爽, 吕丽春, 李虹, 江培洲, 姚开泰. 转染人pIgR的小鼠鼻咽上皮细胞在EBV感染前后基因表达谱差异[J]. 南方医科大学学报, 2004, 24(09): 1001-1005.

WANG Shuang, LÜ Li-chun, LI Hong, JIANG Pei-zhou, YAO Kai-tai. Difference in gene expression profile of human polymeric immunoglobulin receptor-transfected mouse nasopharyngeal epithelial cells before and after EBV infection[J]. Journal of Southern Medical University, 2004, 24(09): 1001-1005.

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