RT-PCR法检测肾癌组织中MN/CAⅨ基因表达

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (08): 925-927.

RT-PCR法检测肾癌组织中MN/CAⅨ基因表达

姜耀东¹, 郑少斌¹, 王战会²

1. 第一军医大学南方医院泌尿外科, 广东, 广州, 510515;
2. 第一军医大学南方医院感染内科实验室, 广东, 广州, 510515

出版日期:2004-08-20 发布日期:2004-08-20
基金资助:
收稿日期:2003-11-29。
作者简介:姜耀东(1974-),男,第一军医大学在读硕士研究生,电话:020-61647097

MN/CAⅨ gene expression in renal cell carcinoma detected by RT-PCR

JIANG Yao-dong¹, ZHENG Shao-bin¹, WANG Zhan-hui²

1. 第一军医大学南方医院泌尿外科, 广东, 广州, 510515;
2. 第一军医大学南方医院感染内科实验室, 广东, 广州, 510515

Online:2004-08-20 Published:2004-08-20

摘要/Abstract

摘要： 目的评价RT-PCR法检测MN/CAⅨ基因在肾癌组织表达的可靠性。方法以5例肾癌和5例正常肾组织为研究对象,提取总RNA,两步法进行RT-PCR反应,用MN/CAⅨ的特异性引物扩增,2%琼脂糖凝胶电泳后观察并测序。结果经过测序证实5例肾癌组织的RT-PCR产物中有4例可见特异的MN/CAⅨ条带,而正常肾组织的RT-PCR产物均无相应的MN/CAⅨ条带。结论 RT-PCR法检测MN/CAⅨ在肾癌组织中的表达是准确、可靠的。

Abstract: Objective To evaluate the reliability of reverse transcriptional (RT)-PCR in detecting MN/CAⅨ gene expression in renal cell carcinoma (RCC). Methods MN/CAⅨ gene expression was detected in 5 RCC tissues and 5 normal renal tissues. The primers of MN/CAⅨ gene were designed for a two-step RT-PCR, and the products examined by 2% agarose gel electrophoresis and identified by sequence analysis. Result Corresponding bands of MN/CAⅨ gene could be seen in RT-PCR products of cases RCC tissue from 4 but not in normal renal tissues. Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.

中图分类号:

R737.11

姜耀东¹, 郑少斌¹, 王战会². RT-PCR法检测肾癌组织中MN/CAⅨ基因表达[J]. 南方医科大学学报, 2004, 24(08): 925-927.

JIANG Yao-dong¹, ZHENG Shao-bin¹, WANG Zhan-hui². MN/CAⅨ gene expression in renal cell carcinoma detected by RT-PCR[J]. Journal of Southern Medical University, 2004, 24(08): 925-927.

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