南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (08): 922-925.

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犬膈神经离断后不同时期呼吸肌能量代谢的改变

田建昌1, 陈钢2, 肖飞3, 马俊杰1, 蔡瑞君2   

  1. 1. 解放军371医院胸心外科, 河南, 新乡, 453000;
    2. 第一军医大学南方医院胸心外科, 广东, 广州, 510515;
    3. 第一军医大学珠江医院胸心外科, 510282
  • 出版日期:2004-08-20 发布日期:2004-08-20
  • 基金资助:
    收稿日期:2003-8-10。
    作者简介:田建昌(1972-),男,第一军医大学硕士毕业,主治医师,E-mail:TitanJC@hotmail.com

Changes of energy metabolism in canine respiratory muscles after phrenic nerve transection

TIAN Jian-chang1, CHEN Gang2, XIAO Fei3, MA Jun-jie1, CAI Rui-jun2   

  1. 1. 解放军371医院胸心外科, 河南, 新乡, 453000;
    2. 第一军医大学南方医院胸心外科, 广东, 广州, 510515;
    3. 第一军医大学珠江医院胸心外科, 510282
  • Online:2004-08-20 Published:2004-08-20

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摘要： 目的 研究犬单侧膈神经离断后不同时期呼吸肌腺嘌呤核苷酸含量变化。方法 选取8只成年健康杂种犬,离断其左侧膈神经,分别取其同侧肋间肌和膈肌组织,用高效液相色谱法测量膈神经离断前及离断后1、2个月呼吸肌中腺嘌呤核苷酸含量。结果 犬肋间肌三磷酸腺苷(ATP)的含量三个时间组相比均无差别;而二磷酸腺苷(ADP)的含量上升,膈神经离断后2月组与离断后1月组相比,有统计学差别(P<0.05);犬肋间肌总腺苷酸(TAN)含量以膈神经离断后1月组为最低,但并无统计学差别;肋间肌腺苷酸能荷(AEC)以膈神经离断后1个月为最低,与离断前组和离断后2月组相比,均有统计学差别(P<0.05)。犬的膈肌ATP、ADP及TAN、AEC含量在膈神经离断后2个月分别为9.05±12.70,2.99±2.57,14.72±13.98,0.57±0.29;与膈神经离断前及离断后1个月相比,有统计学意义(P<0.05)。结论 犬膈神经离断后膈肌能量代谢明显下降,肋间肌有一定程度代偿。

Abstract: Objective To examine the changes in energy metabolism in the respiratory muscles of canines with unilateral phrenic nerve transection using high-performance liquid chromatography (HPLC). Methods The left phrenic nerve of 8 canines was transected and the contents of adenine nucleotide in respiratory muscles were determined by HPLC before and 1 and 2 months after the operation. Results In the intercostal muscles, ATP contents remained almost unchanged during the entire course of the observation, while ADP content was elevated 2 months after the operation as compared with that measured before and 1 month after the operation (P<0.05). One month postoperatively, the content of total adenylic acid (TAN) decreased to the lowest point, but the difference between the measurements was not statistically significant; also at 1 month after the operation, the content of adenylate energy charge (AEC) was the lowest, with statistically significant difference from the measurements before and 2 months after the operation (P<0.05). In the diaphragm, the contents of ATP, ADP, TAN and AEC 2 months postoperatively were 9.05±12.70,2.99±2.57,14.72±13.98,0.57±0.29 from the levels at the other two time points (P<0.05). Conclusion After unilateral phrenic nerve transection in canines, the energy metabolism of the diaphragm declines significantly, whereas that of the intercostal muscle can be compensated to some extent.

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