蚌肉提取物LX01对肺癌细胞株A549的抑制作用

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (08): 917-920.

蚌肉提取物LX01对肺癌细胞株A549的抑制作用

王顺官¹, 李琳²

1. 广东省第二人民医院肿瘤一科, 广东, 广州, 510317;
2. 第一军医大学药理学教研室, 广东, 广州, 510515

出版日期:2004-08-20 发布日期:2004-08-20
基金资助:
收稿日期:2003-10-26。
基金项目:2001年广东省中医药现代化重大科技专项项目(20010305A)
作者简介:王顺官(1967-),男,1990年毕业于第一军医大学临床医学专业,硕士,主治医师

Inhibition of river clam extract LX01 on human lung epithelial carcinoma cell line A549

WANG Shun-guan¹, LI Lin²

1. 广东省第二人民医院肿瘤一科, 广东, 广州, 510317;
2. 第一军医大学药理学教研室, 广东, 广州, 510515

Online:2004-08-20 Published:2004-08-20

摘要/Abstract

摘要： 目的观察蚌肉提取物LX01对肺癌细胞株A549的抑制作用及对血液白细胞数目的影响。方法 MTT法检测不同浓度的LX01对肺癌细胞株A549细胞增殖的抑制率;A549细胞接种BALB/C裸鼠,观察给药后肿瘤的生长情况,高、中、低剂量LX01对荷瘤小鼠的生存时间、瘤重及血液白细胞数目的影响。结果 LX01对A549细胞的增殖有明显的抑制作用,IC50为56.85mg/L,与阴性对照组比差异有显著性(P<0.01);LX01能剂量依赖性的抑制A549接种瘤的生长;LX01中、高剂量可明显延长A549荷瘤小鼠生存时间,高剂量组荷瘤瘤重明显减轻,与阳性对照药环磷酰胺相似,明显高于阴性对照组(P<0.01);与5-FU治疗组相比,LX01各剂量组对A549荷瘤小鼠血白细胞数目无降低作用。结论蚌肉提取物LX01能显著抑制肺癌细胞株A549接种BALB/c裸鼠后的生长,且对免疫系统无明显抑制作用。

Abstract: Objective To explore the inhibition effect of river clam extract LX01 on human lung epithelial carcinoma (A549) in mice. Methods The inhibition ratio of LX01 on A549 was detected by MTT method. A549 was inoculated into BALB/c mice, and 2 days later, LX01 (50, 100, 200 mg/kg·d) were administrated for 15 days. Tumor weight, survival time and leukocyte count were measured. Results Tumor growth was markedly inhibited by LX01 in a dose-depenner. LX01 could significantly extend the survival time compared with the control group (P<0.01), but LX01 did not reduce leukocyte count of mice transplanted with A549. Conclusion LX01 could efficiently inhibit the growth of A549 transplanted into mice and maintain the normal immunological function.

中图分类号:

R730.53

王顺官¹, 李琳². 蚌肉提取物LX01对肺癌细胞株A549的抑制作用[J]. 南方医科大学学报, 2004, 24(08): 917-920.

WANG Shun-guan¹, LI Lin². Inhibition of river clam extract LX01 on human lung epithelial carcinoma cell line A549[J]. Journal of Southern Medical University, 2004, 24(08): 917-920.

参考文献

[1] 李连弟,鲁风珠,张思维,等.中国恶性肿瘤死亡率20年变化趋势和近期预测分析[J].中华肿瘤学杂志,1997,19(1):3-9.Li LD, Lu FZ, Zhang SW, et al. Analysis of mortality rate of tumor and its trend in twenty years in china[J]. Chin J Oncol,1997,19(1):3-9.
[2] Fergusson RJ. Studying lung cancer in the laboratory:l-development of model systems[J]. Thorax, 1987, 42:753-8.
[3] 肖雪明.新型抗肿瘤药物的研究进展[J].国外医学·外科学分册,2001,28(2):70-3.
[4] 何祥梁,蒋小忠,于化鹏,等.旁观者效应在HSV-1-TK/GCV杀灭肺癌细胞A549中的作用[J].第一军医大学学报,2000,20(6):537-8.He XL, Jiang XZ, Yu HP, et al. Role of bystander effect in the cytocidal activity of HSV1-TK/GCV against pulmonarycarcinoma cell A549[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao,2000, 20(6): 537-8.
[5] 刘宝瑞,徐修礼,刘文超,等.4种中药制剂对人肺癌A549细胞增殖的影响[J].中国药理学通报,2002,18(1):94-6.Liu BR, Xu XL, Liu WC, et al. Effects of four extracts if traditional Chinese medicinal materials in the proliferation of human lung cancer A549 cell line[J]. Chin Pharm Bull, 2002, 18(1): 94-6.
[6] 王兴旺,胥彬.抗癌药物筛选模型与筛选方法的研究[J].中国医药工业杂志,1997,28(1):39-43.Wang XW, Xu B. Studies on screening models and methods for anti-tumor drugs[J]. Chin J Pharm, 1997, 28(1): 39-43.
[7] 孔天翰,洞伟华,周永安,等.3种蝎毒抗癌多肽制剂抑制小鼠肺癌及增强免疫的作用比较[J].河南医科大学学报,1999,34(1):55-8.Kong TH, Dong WH, Zhou YA, et al. Comparable study on an tineoplastic and immunity-enhancing effects in mice if three preparation of APBMV[J]. J Henan Med Univ, 1999, 34(1): 55-8.
[8] 朱惠芳,兰岚,赵轼轩,等.A549人肺癌细胞系/615-SCID小鼠转移瘤的生物学特征[J].中国实验动物学报,2003,11(4):199-202.Zhu HF, Lan L, Zhao SX, et al. Characterization of human lung cancer cell line A549 in 615-SCID mice[J]. Acta Lab Anim Sci,2003, 11(4): 199-202.
[9] 袁亚非,王艳辉,刘豫安,等.河蚌提取物增强环磷酰胺造成免疫低下模型小鼠DTH及PFC的作用[J].承德医学院学报,2000,17(3):18-20.Yuan YF, Wang YH, Liu YA, et al. Immunoenhancement effect of extract of river clam on immunosuppression mice induced by cyclophamide[J]. J Chengde Med Coll, 2000, 17(3): 18-20.
[10] Burger JC. Partial purification and characterization of a BCGFII from EL4 culture supernatants[J]. Cell Immunol, 1984, 84: 228-31.
[11] Sawyers CL. Rational therapeutic intervention in cancer: kinases as drug targets[J]. Curr Opin Genet Dev, 2002, 12(1): 111-5.