广东汉族人群HLA-Cw多态性及单体型分析

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (08): 900-903.

广东汉族人群HLA-Cw多态性及单体型分析

马红京¹, 肖露露², 尹晓林¹, 郭坤元¹, 叶欣²

1. 第一军医大学珠江医院血液科, 广东, 广州, 510282;
2. 广州器官移植配型中心, 广东, 广州, 510095

出版日期:2004-08-20 发布日期:2004-08-20
基金资助:
收稿日期:2004-2-26。
基金项目:广州市重大科研项目基金(2000-Z-022-01)
作者简介:马红京(1967-),女,副主任医师,第一军医大学在读博士生,电话:020-61643281
通讯作者:郭坤元,第一军医大学珠江医院血液科,电话:020-61643287

HLA-Cw alleles polymorphism and haplotypes in Guangdong Han population

MA Hong-jing¹, XIAO Lu-lu², Yin Xiao-lin¹, GUO Kun-yuan¹, Ye Xin²

1. 第一军医大学珠江医院血液科, 广东, 广州, 510282;
2. 广州器官移植配型中心, 广东, 广州, 510095

Online:2004-08-20 Published:2004-08-20

摘要/Abstract

摘要： 目的检测广东汉族人群HLA-Cw及HLA-A、B、DRB1基因频率,分析该人群HLA-Cw等位基因多态性及其单体型特点。方法骨髓移植供者185例,抗凝血提取DNA,半量全自动PCR-RSSO分型检测HLA-A、B、Cw、DRB1基因型。结果在分辨水平检出Cw等位基因11个,其中分布频率较高的依次为Cw^*03(0.2580)、Cw^*07(0.1887)、Cw^*01(0.1732)、Cw^*08(0.1070)。统计分析呈现显著连锁不平衡的HLA-Cw-A单体型7个,HLA-Cw-B单体型20个,HLA-Cw-DRB1单体型10个。结论广东汉族群体HLA-Cw基因具有较为丰富的多态性,其与HLA-A、B、DRB1间连锁不平衡单体型具有地区性遗传特征。

Abstract: Objective To analyze the polymorphism and haplotypes of HLA-Cw and detect HLA-A, B, Cw and DRB1 allele frequencies in Guangdong Han population. Method An auto semi-quantitative PCR with reverse sequence-specific oligonucleo- tide was adopted to explore the HLA-A, B, Cw and DRB1 genotypes of 185 bone marrow donors. Result Eleven HLA-Cw alleles were obtained in which Cw^*03 (0.2580), 07 (0.1887), 01 (0.1732), and 08 (0.1071) had much higher allele frequencies. A total of 7 HLA-Cw-A, 20 HLA-Cw-B and 10 HLA-Cw-DRB1 haplotypes were found. Conclusion HLA-Cw alleles have richer polymorphisms and their linkage disequilibrium with HLA-A, B, DRB1 exhibits geographic genetic characteristics.

中图分类号:

R392.13

马红京¹, 肖露露², 尹晓林¹, 郭坤元¹, 叶欣². 广东汉族人群HLA-Cw多态性及单体型分析[J]. 南方医科大学学报, 2004, 24(08): 900-903.

MA Hong-jing¹, XIAO Lu-lu², Yin Xiao-lin¹, GUO Kun-yuan¹, Ye Xin². HLA-Cw alleles polymorphism and haplotypes in Guangdong Han population[J]. Journal of Southern Medical University, 2004, 24(08): 900-903.

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