高效毛细管电泳法测定肤炎康霜中地塞米松磷酸钠的含量

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (07): 839-840.

高效毛细管电泳法测定肤炎康霜中地塞米松磷酸钠的含量

郭丹¹, 陈娜娜², 杨希晓¹, 侯连兵¹

1. 第一军医大学南方医院药学部, 广东, 广州, 510515;
2. 第一军医大学药物研究所, 广东, 广州, 510515

出版日期:2004-07-20 发布日期:2004-07-20
基金资助:
收稿日期:2003-12-9。
作者简介:郭丹(1972- ),男,硕士,主管药师,讲师,电话:020-61641888-87215

Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis

GUO Dan¹, CHEN Na-na², YANG Xi-xiao¹, HOU Lian-bing¹

1. 第一军医大学南方医院药学部, 广东, 广州, 510515;
2. 第一军医大学药物研究所, 广东, 广州, 510515

Online:2004-07-20 Published:2004-07-20

摘要/Abstract

摘要： 目的测定肤炎康霜中地塞米松磷酸钠的含量。方法采用高效毛细管电泳法。毛细管60 cm×75μm、运行缓冲液100 mmol/L四硼酸钠(pH 9.2)、高压进样10s、分离电压25kv、温度为25℃、检测波长为240 nm。结果地塞米松磷酸钠在20～100 μg/ml浓度范围内线性关系良好(r=0.9998),平均回收率为99.75%(n=5,RSD=1.03%)。结论本法简单、快捷、灵敏,可用于肤炎康霜的质量控制。

Abstract: Objective To determine the content of dexamethasone sodium phosphate in Fuyankang cream, a preparation for treatment of various skin conditions. Methods Dexamethasone sodium phosphate content in Fuyankang cream was determined using high-performance capillary electrophoresis (HPCE) at the detection wavelength of 254 nm, under the optimal condition achieved with a fused-silica capillary tube (60 cm×75 μm) and 100 mmol/L sodium tetraborate buffer (pH 9.2) at a constant voltage of 25 kV with a sampling time of 10 s at 25℃. Results The calibration curve displayed good linear relationship within dexamethasone sodium phosphate concentration range of 20 to 100μg/ml (r=0.999 8). The average recovery of dexamethasone sodium phosphate was 99.75% (n=5, RSD=1.03%). Conclusion HPCE is simple, quick and sensitive in determination of dexamethasone sodium phosphate content in Fuyankang cream.

中图分类号:

R927.2

郭丹¹, 陈娜娜², 杨希晓¹, 侯连兵¹. 高效毛细管电泳法测定肤炎康霜中地塞米松磷酸钠的含量[J]. 南方医科大学学报, 2004, 24(07): 839-840.

GUO Dan¹, CHEN Na-na², YANG Xi-xiao¹, HOU Lian-bing¹. Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis[J]. Journal of Southern Medical University, 2004, 24(07): 839-840.

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