南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (07): 832-833.

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胶原支架材料复合膜的制备及其组织培养性能观察

黄汉萍1, 牟善松1, 马安德2   

  1. 1. 暨南大学生物医学工程所, 广东, 广州, 510632;
    2. 第一军医大学中心实验室, 广东, 广州, 510515
  • 出版日期:2004-07-20 发布日期:2004-07-20
  • 基金资助:
    收稿日期:2004-1-19。
    作者简介:黄汉萍(1967- )女,硕士,从事生物材料研究,电话:020-85220469,85676986;E-mail:hhp_xu@yahoo.com.cn.
    通讯作者:通讯作者:牟善松(1962-)男,副研究员,从事生物材料研究,电话:020-85220469,E-mail:tmuss@jnu.edu.cn

Preparation of a composit membrane with collagen scaffold material for tissue culture

HUANG Han-ping1, MOU Shah-song1, MA An-de2   

  1. 1. 暨南大学生物医学工程所, 广东, 广州, 510632;
    2. 第一军医大学中心实验室, 广东, 广州, 510515
  • Online:2004-07-20 Published:2004-07-20

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摘要： 目的 制备纯胶原膜、胶原/HA膜、胶原/甲壳糖膜和胶原/透明质酸/甲壳糖膜(简称胶原复合膜),并研究其组织培养性能。方法 分别测定其体外降解时间和细胞增殖数。结果 (1)降解时间为:纯胶原膜1380 min、胶原/HA膜1376min、胶原复合膜1560min。(2)在胶原/HA膜、胶原复合膜上,增殖细胞的数目一直是最大的。结论 (1)加入甲壳糖有利于降低胶原膜体外降解速度,而透明质酸没有。(2)加入透明质酸有利于细胞在胶原膜上的吸附、生长、增殖,而甲壳糖没有。

Abstract: Objective To prepare a composit membrane with collagen scaffold material for tissue culture. Methods Pure collagen membrane, collagen/hyaluronic acid (HA) membrane, collagen/chitosan membrane and collagen/chitosan/HA membrane (abbreviated as composite collagen membrane) were prepared respectively, and their respective biodegradation time and the number of the cells proliferated on them were examined in vitro. Result The biodegradation time of pure collagen, collagen/HA and composite collagen membranes in vitro was 1380, 1376 and 1560 min respectively, and the number of cells proliferated on the collagen/HA membrane and the composite collagen membrane were greater than that on the pure collagen membrane. Conclusions Addition of chitosan can decrease the biodegradation of the collagen membrane in vitro, while HA can not; HA Can contribute to cell adhesion and proliferation on the collagen membranes, but chitosan does not.

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