第一极体不能精确定位人卵母细胞纺锤体

摘要/Abstract

摘要： 目的观测人卵母细胞纺锤体的确切位置,从而指导今后的生殖医学临床工作。方法应用纺锤体成像系统观测体外成熟及体内成熟组人卵母细胞纺锤体,测量卵子中心、纺锤体与第一极体之间形成的角度。比较两组结果的差异。结果体外成熟组纺锤体与第一极体形成角度和体内成熟组存在显著差异(P=0.006)。结论人卵母细胞的位置并不能准确定位卵母细胞纺锤体的位置。体外成熟组纺锤体与第一极体形成的角度要明显小于体内成熟组。应用纺锤体成像系统可以安全、有效地观察人卵母细胞纺锤体。

Abstract: Objective To observe the precise position of the metaphase Ⅱ spindle for facilitating clinical practice of assisted reproduction. Methods LC-PolScope imaging system was used for observing the spindles in human metaphase Ⅱ oocytes matured in vitro or in vivo, and the angle between the center of the oocyte, the spindle and the first polar body was measured and compared between in vitro and in vivo matured oocytes. Results In vitro and in vivo matured oocytes were significantly different in the angle between the spindle and the first polar body (P=0.006). Conclusions The deviation of the spindle from the polar body in in vitro matured oocyte was significantly smaller than that in in vivo matured oocyte, and the first polar body does not help predict the precise location of the metaphase Ⅱ spindle. The imaging system is safe and effective for observing the spindles.

中图分类号:

R711

朱亮, 孔令红, 李红, 陈士岭, 邢福祺. 第一极体不能精确定位人卵母细胞纺锤体[J]. 南方医科大学学报, 2004, 24(07): 809-811.

ZHU Liang, KONG Ling-hong, LI Hong, CHEN Shi-ling, XING Fu-qi. The first polar body of human oocytes does not help predict the precise location of the spindle[J]. Journal of Southern Medical University, 2004, 24(07): 809-811.

参考文献

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