鬼臼毒素二棕榈酰磷脂酰胆碱前体脂质体的研制及特性观察

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (07): 784-786,788.

鬼臼毒素二棕榈酰磷脂酰胆碱前体脂质体的研制及特性观察

林中方¹, 曾抗¹, 周再高¹, 李国锋², 谢方明¹, 朱晓亮¹, 张三泉¹

1. 第一军医大学南方医院皮肤科, 广东, 广州, 510515;
2. 第一军医大学南方医院药学部, 广东, 广州, 510515

出版日期:2004-07-20 发布日期:2004-07-20
基金资助:
收稿日期:2003-11-30。
基金项目:广东省重点工业攻关项目(2003C104034)
作者简介:林中方(1975- ),男,第一军医大学在读硕士研究生,主治医师,电话:020-61641888-87322,E-mail:achang3333@sohu.com.
通讯作者:曾抗,电话:020-61641981

Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome

LIN Zhong-fang¹, ZENG Kang¹, ZHOU Zai-gao¹, LI Guo-feng², XIE Fang-ming¹, ZHU Xiao-liang¹, ZHANG San-quan¹

1. 第一军医大学南方医院皮肤科, 广东, 广州, 510515;
2. 第一军医大学南方医院药学部, 广东, 广州, 510515

Online:2004-07-20 Published:2004-07-20

摘要/Abstract

摘要： 目的研制鬼臼毒素二棕榈酰磷脂酰胆碱(PPT-DPPC)前体脂质体,提高PPT-DPPC脂质体的稳定性。方法采用冷冻干燥法,选择海藻糖做冻干剂制备PPT-DPPC前体脂质体,并考察PPT-DPPC前体脂质体水合后的形态、粒径分布、包封率和稳定性。结果 PPT-DPPC前体脂质体经水合后在电镜下呈多层多室脂质体,脂质体粒径分布均匀,平均粒径(1.45±0.38)μm,药物包封率为72.3%。分别在4℃、20℃、40℃贮存1、3、6个月,脂质体形态、粒径及包封率均无明显变化。结论冷冻干燥法制备PPT-DPPC前体脂质体,方法简便易行,制剂粒径分布均匀、包封率高、有良好稳定性。

Abstract: Objective To prepare podophyllotoxin-dipalmitoylphosphatidylcholine (PPT-DPPC) proliposomes (PPT-DPPC-PL) for improvement of the stability of PPT-DPPC liposome. Methods Freeze-drying method was used to prepare PPT-DPPC-PL, and the particle morphology, size range, encapsulation efficiency and stability of PPT-DPPC liposome were investigated. Results After hydration of PPT-DPPC-PL, PPT-DPPC liposome appeared multivesicular under electron microscope and the particles were distributed homogenously with an average particle size of 1.45±0.38 μm. The encapsulation efficiency of PPT was 72.3%, and alter storage at 4 to 40℃ for Ⅰ to 6 months, the proliposome remained stable. Conclusion The prepared PPT-DPPC-PL particles by freeze-drying method are evenly distributed. The preparation method is relatively simple with higher embedding ratio and better stability.

中图分类号:

R979.19
R986

林中方¹, 曾抗¹, 周再高¹, 李国锋², 谢方明¹, 朱晓亮¹, 张三泉¹. 鬼臼毒素二棕榈酰磷脂酰胆碱前体脂质体的研制及特性观察[J]. 南方医科大学学报, 2004, 24(07): 784-786,788.

LIN Zhong-fang¹, ZENG Kang¹, ZHOU Zai-gao¹, LI Guo-feng², XIE Fang-ming¹, ZHU Xiao-liang¹, ZHANG San-quan¹. Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome[J]. Journal of Southern Medical University, 2004, 24(07): 784-786,788.

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