抗吗啡抗体的制备及其特性评价

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (06): 673-676.

抗吗啡抗体的制备及其特性评价

李琳¹, 赵文忠², 杨淑琴¹, 富宁², 徐江平¹

1. 第一军医大学药理教研室, 广东, 广州, 510515;
2. 第一军医大学免疫教研室, 广东, 广州, 510515

出版日期:2004-06-20 发布日期:2004-06-20
基金资助:
收稿日期:2004-2-28。
基金项目:广州市科技攻关项目(2002Z3-E4102)
作者简介:李琳(1975- ),女,第一军医大学在读硕士研究生,电话:020-61648235,E-mail:li751in@fimmu.com.
通讯作者:徐江平,电话:020-61648236,E-mail:jpx@fimmu.com.

Preparation and characterization of anti-morphine antiserum

LI Lin¹, ZHAO Wen-zhong², YANG Shu-qin¹, FU Ning², XU Jiang-ping¹

1. 第一军医大学药理教研室, 广东, 广州, 510515;
2. 第一军医大学免疫教研室, 广东, 广州, 510515

Online:2004-06-20 Published:2004-06-20

摘要/Abstract

摘要： 目的评价吗啡及琥珀酰化吗啡半酯两种交联物作为抗原免疫小鼠而产生的免疫效果及其中和阿片类物质的能力。方法吗啡及吗啡半酯在碳二酰亚胺催化下分别与Blue carrier载体蛋白交联,用其免疫小鼠,ELISA检测免疫小鼠血清中抗体滴度及抗体6个月内效价维持状况,通过竞争性抑制实验检测抗体对吗啡及海洛因的体外结合能力,与其他阿片及非阿片类化学物质的交叉反应。结果两种交联物免疫小鼠第9周血清中平均抗吗啡抗体滴度均超过1:8 000,其中M-6-S-Blue组效价强度较M-Blue组高,且效价维持时间较久;两组抗体对较低浓度吗啡及海洛因的抗血清抑制率均达50%以上,抑制率与吗啡或海洛因的浓度呈剂量依赖性。结论两种载体蛋白交联物均能诱导小鼠产生高滴度的抗吗啡抗体,其体内及体外实验观察均能产生中和吗啡的作用,提示其对控制复吸将有较好的应用前景。

Abstract: Objective To prepare the holoantigens of morphine and morphine-6-succinyl, and to evaluate the efficacy of both of the haptens in eliciting immune responses and their effects on morphine withdrawal symptoms in male mice. Methods Morphine-6-succinyl was prepared using acid anhydride mixture, and conjugated, along with morphine, with Blue carrier in the presence of carbodi imide. Immunization of mice with both the holoantigens and Freund adjuvant was performed, followed by determination of the antibody titer in the mouse serum with competitive ELISA and morphine dependence evaluation. Results The titers of the antibodies exceeded 1:8 000 in the serum of mice immunized with both holoantigens, and morphine-6-succinyl induced a higher titer that was maintained for a longer period. The inhibition rates of the antisera to morphine and morphine-6-auccinyl had both reached 50%, and increased in a dose-dependent manner. Conclusion Both morphine carrier conjugates can elicit high-titer antibody response in mice and relieve morphine addiction.

中图分类号:

R996

李琳¹, 赵文忠², 杨淑琴¹, 富宁², 徐江平¹. 抗吗啡抗体的制备及其特性评价[J]. 南方医科大学学报, 2004, 24(06): 673-676.

LI Lin¹, ZHAO Wen-zhong², YANG Shu-qin¹, FU Ning², XU Jiang-ping¹. Preparation and characterization of anti-morphine antiserum[J]. Journal of Southern Medical University, 2004, 24(06): 673-676.

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