Abstract: Objective To explore the effect of the tri-cuff vascular anastomosis for simplifying the surgical procedure and improving the success rate. Method Sixty recipient Wistar rats were randomized into experimental group and the control group, the former was subjected to the tri-cuff vascular anastomosis for heterotopic small bowel transplantation while the latter had vascular anastomosis using classical methods. The effects of the two methods for anastomosis were compared. Results The tri-cuff vascular anastomosis was superior to the classical methods in view of the total operation time, arterial anastomosis time, and hot-ischemia time of the small bowel graft. Conclusion Compared with the classical methods, the tri-cuff vascular anastomosis is safer and time-saving.