海水型呼吸窘迫综合征犬模型的实验研究

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (06): 665-669.

海水型呼吸窘迫综合征犬模型的实验研究

傅卫军, 古妙宁, 陈仲清, 肖金仿, 黄毅然, 周伟, 陈晔明

第一军医大学南方医院SICU海水研究组, 广东, 广州, 510515

出版日期:2004-06-20 发布日期:2004-06-20
基金资助:
收稿日期:2003-12-9。
基金项目:广东省自然科学基金(2003C33715)
作者简介:傅卫军(1974- ),男,第一军医大学在读硕士研究生,医师、助教,电话:020-61641888-87271.

Respiratory distress syndrome induced by immersion in seawater:a study of canine models

FU Wei-jun, GU Miao-ning, CHEN Zhong-qing, XIAO Jin-fang, HUANG Yi-ran, ZHOU Wei, CHEN Ye-ming

第一军医大学南方医院SICU海水研究组, 广东, 广州, 510515

Online:2004-06-20 Published:2004-06-20

摘要/Abstract

摘要： 目的构建海水型呼吸窘迫综合征(SW-RDS)的犬模型,为研究海水淹溺后的呼吸窘迫综合征的病理表现和治疗提供平台。方法选择12只健康犬随机分为2组,即对照组(n=4)和模型组(n=8)。对比两组血流动力学、血气分析、电解质等指标和肺组织细胞学变化,并检测支气管肺泡液和血液乳酸脱氢酶(LDH-L)、碱性磷酸酶(ALP)浓度。结果与对照组相比,模型组出现明显的血流动力学、血气和乳酸的变化,病理结果显示肺组织损伤明显,LDH-L和ALP持续显著升高。上述变化符合SW-RDS的诊断标准。结论 SW-RDS犬模型能够反映海水淹溺后的病理生理变化规律和SW-RDS的病理特征。

Abstract: Objective To study the mechanism of respiratory distress syndrome (RDS) induced by immersion in seawater to provide experimental evidence for its treatment. Methods Twelve normal hybrid dogs were randomly assigned into control group (n=4) and SDS model group (n=8). The changes in blood dynamics, blood gas analysis and histological changes in the lung tissues were compared between the 2 groups. The concentration of lactic dehydrogenase (LDH-L) and alkaline phospha-tase (ALP) in the bronchoalveolar fluid and blood of the dogs in the model group were tested. Results The blood dynamics, blood gas analysis and histology of the dogs in the model group were significantly different from those in the control group, and LDH-L and ALP levels increased significantly in the bronchoalveolar fluid of the model group. Conclusion Seawater aspiration into the lungs may lead to RDS, and the canine models used in this study may help explore the mechanism and management of RDS induced by immersion in seawater.

中图分类号:

R563.8
R-332

傅卫军, 古妙宁, 陈仲清, 肖金仿, 黄毅然, 周伟, 陈晔明. 海水型呼吸窘迫综合征犬模型的实验研究[J]. 南方医科大学学报, 2004, 24(06): 665-669.

FU Wei-jun, GU Miao-ning, CHEN Zhong-qing, XIAO Jin-fang, HUANG Yi-ran, ZHOU Wei, CHEN Ye-ming. Respiratory distress syndrome induced by immersion in seawater:a study of canine models[J]. Journal of Southern Medical University, 2004, 24(06): 665-669.

参考文献

[1] Golden FS,Tipton MJ,Scott RC.Immersion,near-drowning and drowning[J].BrJAnesth,1997,79(2):214-5.
[2] Szpilmam D.Near drowning classification:a proposal to stratify mortality based on the analysis of 1 831 cases[J].Chest,1997,112(3):660-5.
[3] Bernard GR,Artigas A,Brigham KL,et al.The American-European Consensus Conference on acute respiratory distress syndrome:Definitions,mechanisms,relevant outcomes,and clinical trial coordination[J].AmJRespir Crit Care Med,1994,149(3 Pt 1):818-24.
[4] 古妙宁,肖金仿,黄毅然,等.海水直接肺损伤的犬模型研究[J].第一军医大学学报,2003,23(3):201-5.Gu MN,XiaoJF,Huang YR,et al.Study of direct lung injury by seawater in canine models [J].JFirst Mil Med Univ/Di YiJun Yi Da Xue Xue Bao,2003,23(3):201-5.
[5] 薏文度,刘新卷,傅更锋,等.兔海水淹溺肺水肿发生机制的实验研究[J].生物医学工程学杂志,2000,17(2):196-201.Dong WD,Liu XJ,Fu GF,et al.Study on the mechanism of pulmonary edema after seawater drowning in rabbit[J].JBiomed Eng,2000,17(2):196-201.
[6] 黄毅然,古妙宁,肖金仿,等.早期肺灌洗治疗犬海水淹溺肺损伤的实验研究[J].第一军医大学学报,2003,23(10):1062-5.Huang YR,Gu MN,XiaoJF,et al.Treatment of acute lung injury due to sea water aspiration in dogs by early massive bronchoalveolar lavage:an experimental study [J].JFirst Mil Med Univ/Di YiJun Yi Da Xue Xue Bao,2003,23(10):1062-5.
[7] 袁世荧,卫富强,姚尚龙.HHS对急性呼吸窘迫综合征兔动脉血气变化的影响[J].华中医学杂志,2001,25(6):297-8.Yuan SY,Wei FQ,Yao SL.The effect of HHS on the changes in blood gas in the rabbit with ARDS[J].Cent Chin MedJ,2001,25(6):297-8.
[8] 曹相原,马少林,段风梅,等.动脉血乳酸水平与血清组织释放酶在危重病患者中的变化[J].中国医师杂志,2001,3(4):248-50.Cao XY,Ma SL,Duan FM,et al.The changes of arterial blood lactate and serum tissue release enzymes (LDH,AKP,CK) in the critical illness and their correlation [J].JChin Phys,2001,3(4):248-50.
[9] 朱光发,钮善福,章红志,等.急性肺损伤患者血清肺表面活性物质蛋白A的变化及其意义[J].中国危重病急救医学,2000,12(1):15-7.Zhu GF,Niu SF,Zhang HZ,et al.Determination of serum pulmonary surfactant protein A and its significance in patients with acute lung injury[J].Chin Crit Care Med,2000,12(1):15-7.
[10] Avery ME,MeadJ.Surface properties in relation to atelectasis and hyaline membrane disease[J].AmJDis Child,1959,97(5 Part 1):517-23.
[11] Ashbaugh DG,Bigelow DB,Petty TL,et al.Acute respiratory distress in adults[J].Lancet,1967,2(7511):319-23.