南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (05): 572-573.

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老年心脏病患者血清柯萨奇病毒B特异性抗体和核酸的检测

孙芸芸1, 蒋文玲2, 罗宪玲2   

  1. 1. 广东省心血管病研究所, 广东, 广州, 510100;
    2. 广东省老年医学研究所, 广东, 广州, 510080
  • 出版日期:2004-05-20 发布日期:2004-05-20
  • 基金资助:
    收稿日期:2004-2-16。
    基金项目:广东省科技攻关项目(99MO4608G)
    作者简介:孙芸芸(1952- ),女,广东省心血管病研究所副主任医师,电话:020-83827812-41241

Detection of serum coxsackievirus B-specific antibody and nucleic acid in aged patients with cardiopathy

SUN Yun-yun1, JIANG Wen-lin2, LUO Xian-ling2   

  1. 1. 广东省心血管病研究所, 广东, 广州, 510100;
    2. 广东省老年医学研究所, 广东, 广州, 510080
  • Online:2004-05-20 Published:2004-05-20

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摘要： 目的 了解柯萨奇B组病毒(CVB)在老年人中的感染状况,探讨CVB感染与心血管疾病的内在关系。方法 采用间接酶联免疫吸附试验(ELISA)和反转录PCR方法对230例老年人进行了CVB IgG 抗体和核酸检测。结果 心脏病组CVB IgG抗体阳性率为43.61%(75/172),对照组抗体阳性率为15.52%(9/58),两者比较有显著性差异(P<0.01)。CVB RNA 检测结果显示,心脏病组阳性率为20.93%(36/172),对照组为3.45%(25/58),两者比较有显著性差异(P<0.01)。结论 在老年心脏病患者中CVB感染较为普遍,应引起重视。

Abstract: Objective To understand the status of coxsackievirus B (CVB) infection in senior subjects and explore the association of the infection with cardiovascular diseases. Method By means of ELISA and RT-PCR, CVB IgG antibody and nucleic acid were detected in 172 patients with cardiopathy and 58 control subjects. Results The positivity rate of CVB IgG antibody was 43.61% (75/172) in the patient group and 15.52% (9/58) in the control group, showing obvious difference between the two groups. CVB RNA tests revealed a CVB RNA-positivity rate of 20.93% (36/172) in the former group and 3.45%(2/58) in the latter, with also a significant difference (P<0.01). Conclusion CVB infection is prevalent in aged patients with heart disease, which should be given due attention to.

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