成肌调节因子MyoD与myogenin在肌肉损伤修复过程的动态变化

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (05): 542-545.

成肌调节因子MyoD与myogenin在肌肉损伤修复过程的动态变化

曾缨¹, 张成¹, 刘克玄², 李才明¹, 冯善伟¹, 李群¹, 柳太云¹, 黄文¹

1. 中山大学附属第一医院, 神经内科, 广东, 广州, 510080;
2. 中山大学附属第一医院, 麻醉科, 广东, 广州, 510080

出版日期:2004-05-20 发布日期:2004-05-20
基金资助:
收稿日期:2004-3-9。
基金项目:国家自然科学基金(30170337);广东省自然科学基金(970061);卫生部临床重点项目(2001321)
作者简介:曾缨(1969- ),女,1993年本科毕业于中山医科大学,现为在职在读博士研究生,主治医师、讲师,电话:020-87755766-8282.E-mail:drzengying@21cn.com
通讯作者:张成(1957- ),男,博士,电话:87332387;E-mail:czym@gzsums.edu.cn

Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury

ZENG Ying¹, ZHANG Cheng¹, LIU Ke-xuan², LI Cai-ming¹, FENG San-wei¹, LI Qun¹, LIU Tai-yun¹, HUANG Wen¹

1. 中山大学附属第一医院, 神经内科, 广东, 广州, 510080;
2. 中山大学附属第一医院, 麻醉科, 广东, 广州, 510080

Online:2004-05-20 Published:2004-05-20

摘要/Abstract

摘要： 目的探讨成肌调节因子MyoD与myogenin在肌肉损伤修复过程中的动态表达。方法用盐酸布比卡因局部注射制作肌肉损伤模型,在损伤后不同时间点取腓肠肌行冰冻切片,HE染色显示损伤肌肉的病理变化,用SABC法检测MyoD与myogenin的表达。结果 MyoD在肌肉损伤后18 h开始表达,48 h达高峰;myogenin在肌肉损伤后24 h开始表达,72 h达高峰。结论 MyoD与myogenin在肌肉损伤后的再生修复过程中起作用,可作为鉴定肌肉前体细胞和反映肌肉再生的指标。

Abstract: Objective To observe the dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during the repair of injured muscle. Methods Muscular injury model was established by local injection of bupivacain, and at different time points following the injection, the gastrocnemius muscles were collected for preparation of cryosections. HE staining was performed for examination of the pathological changes in the injured muscles, and the expressions of MyoD and myogenin were detected by SABC. Results MyoD-positive nuclei began to appear 18 h after muscle injury, reaching the peak till 48 h after the injury. Myogenin-positive nuclei appeared 24 h after muscle injury and peaked at 72 h. Conclusions MyoD and myogenin play a role in muscle regeneration after muscle injury, and they may serve as the indexes to identify muscle precursor cells and mark muscle regeneration process.

中图分类号:

R746.2

曾缨¹, 张成¹, 刘克玄², 李才明¹, 冯善伟¹, 李群¹, 柳太云¹, 黄文¹. 成肌调节因子MyoD与myogenin在肌肉损伤修复过程的动态变化[J]. 南方医科大学学报, 2004, 24(05): 542-545.

ZENG Ying¹, ZHANG Cheng¹, LIU Ke-xuan², LI Cai-ming¹, FENG San-wei¹, LI Qun¹, LIU Tai-yun¹, HUANG Wen¹. Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury[J]. Journal of Southern Medical University, 2004, 24(05): 542-545.

参考文献

[1] Nonaka I, Takagi A, Ishiura S, et al. Pathophysiology of muscle fiber necrosis induced by bupivacaine hydrochloride (Marcaine) [J].Acta Neuropathol (Berl), 1983, 60(2): 167-74.
[2] Akiyama C, Kobayashi S, Nonaka I. Comparison of behavior in muscle fiber regeneration after bupivacaine hydrochloride- and acid anhydride-induced myonecrosis[J]. Acta Neuropathol, 1992, 83(5):584-9.
[3] 庞建新,程希远,吴曙光.转录因子Sp1和Sp3对JurkatT细胞端粒酶活性的调节作用[J].第一军医大学学报,2002,22(6):481-5.Pang JX, Cheng XY, Wu SG, et al. Regulation of telomerase activity in Jurkat T cells by transcription factors Spl and Sp3 [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2002, 22(6): 481-5.
[4] 奚政君,张忠德,祝明洁,等32例小儿横纹肌肉瘤生肌因子的表达[J].上海第二医科大学学报,2002,22(3):263-5.Xi ZJ, Zhang ZD, Zhu M J, et al. Expression and significance of myogenic factors in 32 children with rhadomyosarcoma [J]. Acta Univ Med Sec Shanghai, 2002, 22(3): 263-5.
[5] Weintraub H, Davis R, Tapscott S, et al. The MyoD gene family:Nodal point during specification of the muscle cell lineage [J]. Science, 1991,251(4995): 761-6.
[6] Sassoon DA. Myogenic regulatory factors: Dissecting their role and regulation during vertebrate embryogenesis[J]. Dev Biol, 1993, 156(1): 1123.
[7] Sabourin LA, Rudnicki MA. The molecular regulation of myogenesis[J]. Clin Genet, 2000, 57(1): 16-25.
[8] Molkentin J D, Olson E N. Combinatorial control of muscle development by basic helix-loop-helix and MADS-box transcription factors [J]. Proc Natl Acad Sci USA, 1996, 93(18): 9366-73.
[9] 魏宽海,金丹,裴国献.逆转录病毒介导人重组骨形态发生蛋白基因转染骨骼肌卫星细胞mRNA的表达[J].第一军医大学学报,2002,22(4):312-5.Wei KH, Jing D, Pei GX. mRNA expression of recombinant human bone morphogenetic protein 7 in skeletal muscle satellite cells with retroviral vector- mediated rhBMP7 gene transfection [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2002, 22(4): 312-5.
[10] Jin Y, Murakami N, Saito Y, et al. Expression of MyoD and myogenin in dystrophic mice, mdx and dy, during regeneration[J]. Acta Neuropathol, 2000, 99(6): 619-27.
[11] Grounds MD, Garrett KL, Lai MC, et al. Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes[J]. Cell Tissue Res, 1992, 267(1): 99-104.
[12] Koishi K, Zhang M, McLennan IS, et al. MyoD protein accumulates in satellite cells and is neurally regulated in regenerating myotubes and skeletal muscle fibers[J]. Dev Dyn, 1995, 202(2): 244-54.
[13] Kami K, Noguchi K, Senba E. Localization of myogenin, c-fos,c-jun, and muscle-specific gene mRNAs in regenerating rat skeletal muscle[J]. Cell Tissue Res, 1995, 280(1): 11-9.