南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (04): 456-458.

• • 上一篇    下一篇

高效毛细管电泳法测定蒲公英及妇洁灌肠液中咖啡酸的含量

晏媛, 许重远, 陈志良, 谭亚非, 郭丹   

  1. 第一军医大学南方医院药学部, 广东, 广州, 510515
  • 出版日期:2004-04-20 发布日期:2004-04-20
  • 基金资助:
    收稿日期:2004-2-25。
    作者简介:晏媛(1972-),女,现为第一军医大学在读硕士研究生,主管药师,研究方向:药物分析,电话:020-61641888-87215

High-performance capillary electrophoresis for determining caffeic acid content in Fujie enema and Taraxacum mongolicum Hand.-Mazz

YAN Yuan, XU Zhong-yuan, CHEN Zhi-liang, TAN Ya-fei, GUO Dan   

  1. 第一军医大学南方医院药学部, 广东, 广州, 510515
  • Online:2004-04-20 Published:2004-04-20

PDF

1019

摘要： 目的 采用高效毛细管电泳法对妇洁灌肠液及蒲公英原药材中所含咖啡酸进行含量测定,尝试从原药材到成品二个层面来控制妇洁灌肠液的质量。方法 高效毛细管电泳法。电泳条件为:石英毛细管柱(75 μm×60 cm);运行缓冲液为20 mmol/L的硼砂溶液(25 ℃时pH=9.18);分离电压12 kV;真空进样时间为5 s;温度为25 ℃,紫外检测波长为313 nm。结果 咖啡酸在10~100 μg/ml范围内呈良好的线性关系(r=0.999 2)。平均回收率大于95%;RSD均小于3% (n=3)。结论 本法简单、快捷、灵敏,可用于蒲公英及妇洁灌肠液的质量控制。

Abstract: Objective To determine caffeic acid content in Fujie enema and the crude traditional Chinese herbal drug Taraxacum mongolicum Hand.-Mazz. for controlling the quality of the enema at the levels of both the crude drugs and the final product. Methods Caffeic acid content in both the enema and the crude drug was determined at 313 nm Using high- performance capillary electrophoresis (HPCE), under the optimized conditions achieved with a fused-silica capillary tube (75 μm×0 cm) and 20 mmol/L borate running buffer (pH=9.18) at a constant voltage of 12 kV and a sampling time of 5 s at 25 ℃. Results The calibration curve displayed good linear relationship within caffeic acid concentration range of 10 to 100 μg/ml (r=0.999 2). The regression equation was Y=1002.45X-327.87, and the average recovery was more than 95%. Conclusion HPCE is simple, rapid and sensitive in separation and determination of caffeic acid in Fujie enema and the crude drug of Taraxacum mongolicum Hand.-Mazz.

中图分类号: