南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (04): 419-422.

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脂质体鬼臼毒素壳聚糖膜剂制备及膜剂中鬼臼毒素含量的测定

曾抗1, 朱晓亮1, 李国锋2, 周再高1   

  1. 1. 第一军医大学南方医院皮肤科, 广东, 广州, 510515;
    2. 第一军医大学南方医院药学部, 广东, 广州, 510515
  • 出版日期:2004-04-20 发布日期:2004-04-20
  • 基金资助:
    收稿日期:2003-12-6。
    基金项目:国家自然科学基金(39970680);广东省重点科技攻关计划项目(C11704)
    作者简介:曾抗(1959-),男,教授,硕士研究生导师,E-mail:npff@fimmu.com

Preparation of liposome podophyllotoxin chitosan film and determination the podophyllotoxin content in the film by ultraviolet spectrophotometry

ZENG Kang1, ZHU Xiao-liang1, LI Guo-feng2, ZHOU Zai-gao1   

  1. 1. 第一军医大学南方医院皮肤科, 广东, 广州, 510515;
    2. 第一军医大学南方医院药学部, 广东, 广州, 510515
  • Online:2004-04-20 Published:2004-04-20

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摘要： 目的 筛选出脂质体鬼臼毒素壳聚糖膜剂制备的最佳配方,制备脂质体鬼臼毒素壳聚糖膜剂并测定膜剂中鬼臼毒素的含量。方法 选择制备壳聚糖膜剂所需的壳聚糖、醋酸、明胶作为三个因素,脂质体鬼臼毒素作为空白因素,每个因素三水平进行正交实验设计,根据正交实验设计的结果制备不同配方的膜剂,将膜剂的粘附性、溶解性作为评价指标进行综合评价,确定膜剂制备的最佳配方。采用紫外分光光度法对所制备膜剂中鬼臼毒素的含量进行测定,检测波长为292 nm。结果 对各配方膜剂的综合评价进行统计分析,以2%壳聚糖、1%醋酸、2%明胶、2.8%脂质体鬼臼毒素为配方制备的脂质体鬼臼毒素壳聚糖膜剂的综合评价最高;紫外分光光度法检测结果显示鬼臼毒素在5~25 μg/ml范围内线性关系良好(r=0.999 89),平均回收率为99.7%(n=5),相对标准误差(RSD)=1.11,膜剂中鬼臼毒素平均含量为10.46%,RSD=1.61%。结论 根据正交实验结果制备的膜剂,在粘附性、溶解性方面均符合要求,紫外分光光度法测定脂质体鬼臼毒素壳聚糖膜剂中鬼臼毒素含量快捷、准确,膜剂中鬼臼毒素的含量基本符合要求,鬼臼毒素在膜剂中的分布较为均一。

Abstract: Objective To optimize the formula of liposome podophyllotoxin chitosan film and accurately determine the podophyllotoxin content in the film. Methods Liposome podophyllotoxin chitosan films were prepared according to different formula derived from the results of orthogonal design by selecting chitosan, acetic acid and gelatin as the 3 factors for preparation, each factor including 3 levels with liposome podophyllotoxin as the blank factor. Comprehensive evaluation of the formulas was performed on the basis of the conglutination and solubility of the prepared film to select the optimal formula. The content of podophyllotoxin was determined by ultraviolet spectrophotometry at 292 nm. Results The conglutination and solubility were optimal of the film prepared from 2% chitosan, 1% acetic acid, 2% gelatin and 2.8% liposome podophyllotoxin chitosan, showing good linearity within podophyllotoxin concentration range of 5-25 μg/ml (r=0.999 89) with an average recovery rate of 99.7% (n=5) and RSD of 1.11%. Conclusion The film prepared according to the orthogonal design results conforms to the requirements in terms of conglutination and solubility, and the method we adopted is fast and accurate, yielding qualified film with censored podophyllotoxin content that distributes homogeneously.

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