肿瘤坏死因子对滋养细胞侵袭性的影响

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (04): 397-399,407.

肿瘤坏死因子对滋养细胞侵袭性的影响

陈慧, 庞战军, 陈士岭, 邢福祺

第一军医大学南方医院妇产科, 广东, 广州, 510515

出版日期:2004-04-20 发布日期:2004-04-20
基金资助:
收稿日期:2003-9-6。
基金项目:973国家重点基础研究发展规划(G1999055903)
作者简介:陈慧(1971-),女,1994年毕业于第一军医大学临床医学系,现在读硕士研究生,主治医师,电话:020-61641908,E-mail:Chenhui175@163.com

Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts

CHEN Hui, PANG Zhan-jun, CHEN Shi-ling, XING Fu-qi

第一军医大学南方医院妇产科, 广东, 广州, 510515

Online:2004-04-20 Published:2004-04-20

摘要/Abstract

摘要： 目的探讨外源性肿瘤坏死因子(TNF-α)及其多克隆抗体对原代滋养细胞侵袭力的影响。方法采用逆转录多聚酶链反应(RT-PCR)方法,检测TNF-α、anti TNF-α、TNF-α+anti TNF-α对原代滋养细胞基质金属蛋白酶(MMP-2)及其组织抑制因子(TIMP-2)表达的影响。结果原代滋养细胞表达MMP-2,经过5 ng/ml的TNF-α、TNF-α+antiTNF-α分别刺激培养原代滋养细胞48 h后,细胞MMP-2的表达显著性提高(P<0.001),50 ng/ml的antiTNF-α刺激培养后MMP-2的表达显著性降低(P<0.001);各组间MMP-2的表达存在显著性差异(P<0.001),但是均未见明显的TIMP-2的表达。结论外源性细胞因子TNF-α可以增强滋养细胞的侵袭力,多克隆抗体antiTNF-α则降低其侵袭力。

Abstract: Objective To observe the effect of exogenous tumor necrosis factor alpha (TNF-α) and its polyclonal antibody (anti-TNF-α) on the invasiveness of human trophoblasts in vitro. Methods The effect of exogenous TNF-αand anti-TNF-αon the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human trophoblastic cells was investigated with reverse transcription (RT)-PCR (RT-PCR). Results The trophoblasts cultured in vitro expressed MMP-2 but not TIMP-2. After incubation of the trophoblasts with 5 ng/ml TNF-αand TNF-αplus anti-TNF-αrespectively for 48 h, significant elevation of MMP-2 expression was induced, while the reverse occurred following incubation of the cells with 50 ng/ml anti-TNF-α. None of the treatments induced TIMP-2 expression. Conclusion Exogenous TNF-αmay promote the invasiveness of the trophoblasts, which can be inhibited by anti-TNF-α.

中图分类号:

陈慧, 庞战军, 陈士岭, 邢福祺. 肿瘤坏死因子对滋养细胞侵袭性的影响[J]. 南方医科大学学报, 2004, 24(04): 397-399,407.

CHEN Hui, PANG Zhan-jun, CHEN Shi-ling, XING Fu-qi. Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts[J]. Journal of Southern Medical University, 2004, 24(04): 397-399,407.

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