南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (04): 389-391.

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睾酮对人单核细胞雄激素受体蛋白表达的影响

刘长青1, 吴赛珠1, 王子东2, 赖文岩1, 孙飞1   

  1. 1. 第一军医大学南方医院心内科, 广东, 广州, 510515;
    2. 解放军第533医院内一科, 云南, 昆明, 650000
  • 出版日期:2004-04-20 发布日期:2004-04-20
  • 基金资助:
    收稿日期:2003-10-28。
    基金项目:国家重点研究发展规划(“973”课题)(G200057008)
    作者简介:刘长青(1974-),研究生,医师,E-mail:liucqing@fimmu.com

Effect of testosterone on expression of androgen receptor in human monocytic cell line THP-1

LIU Chang-qing1, WU Sai-zhu1, WANG Zi-dong2, LAI Wen-yang1, SUN Fei1   

  1. 1. 第一军医大学南方医院心内科, 广东, 广州, 510515;
    2. 解放军第533医院内一科, 云南, 昆明, 650000
  • Online:2004-04-20 Published:2004-04-20

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摘要： 目的 研究不同浓度的睾酮对人单核细胞中雄激素受体(AR)蛋白表达的影响。方法 对培养的人单核细胞株THP-1予不同浓度的睾酮刺激,采用Western blotting检测细胞中AR蛋白表达量,进行半定量比较。结果 人的单核细胞中存在AR。AR表达量随睾酮浓度的增加而减少。加用选择性受体阻滞剂氟他胺可使这种作用完全阻滞。结论 AR表达量随睾酮浓度的增加而减少,并具有一定的时间、剂量依赖性。

Abstract: Objective To investigate the effect of testosterone on the expression of androgen receptor (AR) in human monocytic cell line THP-1. Methods Cultured THP-1 cells were stimulated by testosterone with different concentrations and the AR protein was examined by Western blotting. Results AR expression was observed in unstimulated THP-1 cells and the quantity of AR expression was reduced by testosterone treatment in a dose-dependent manner. A selective AR blocker com- pletely abolished this inhibitory effect of testosterone on AR expresison. Conclusion Testosterone simulation time- and dose- dependently reduces AR expression in THP-1 cells.

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