广州地区人类杯状病毒感染的初步研究

摘要/Abstract

摘要： 目的了解广州地区儿童中是否存在人类杯状病毒(HuCV)感染并初步探讨其感染水平。方法用ELISA法和RT-PCR分别检测266份不同年龄段儿童血清中诺沃克样病毒(NLV)的rNV、rMX和r387特异性IgG抗体和粪便标本中的HuCV RNA并将扩增目的产物同基因文库中的病毒序列进行比较分析。结果血清中的rNV、rMX和r387IgG抗体阳性率分别为68.0%、51.5%和71.1%。腹泻高峰期收集的100份散发非菌性胃肠炎病例粪便标本中检出HuCV4株,均为NLV。在广州某学校暴发型非细菌性胃肠炎患者的10份标本中检出NLV3株。结论广州地区存在NLV引起的散发和暴发性非菌性胃肠炎,当地儿童在婴幼儿期即获得NLV感染,感染率有随年龄增加而升高的趋势。

Abstract: Objective To investigate of human calicivirus (HuCV) infection in the children of Guangzhou, the capital of Guangdong Province at China, and conduct preliminary study of the prevalence of the virus. Methods Serum specimens from 266 children of different ages were tested by an indirect enzyme-linked immunosorbent assay (ELISA) for specific IgG against recombinant Norwalk virus particles (rNV), recombinant Mexico virus partides(rMX) and recombinat 387 Virus particles(r387), respectively, and fecal specimens were collected for detection of HuCV RNA using reverse transcriptional (RT)-PCR assisted by sequence analysis on the basis of comparison of the amplified product with the sequence entries in GenBank. Results The seroprevalence was 68.0% for rNV, 51.5% for rMX and 71.1% for r387 in the cohort of children enrolled in this study. Norwalk-like viruses (NLVs) were detected in 4 out of the 100 fecal specimens collected from cases of nonbacterial gastroenteritis during the peak season of diarrhea, and also in 3 of the 10 fecal specimens collected during the outbreak of diarrhea in an elementary school. Conclusions Sporadic and fulminant NLV infections are present in Guangzhou. The children have the infection in their infancy, and the infection rate increases sharply with age.

中图分类号:

R373

戴迎春¹, 聂军¹, 刘翼¹, 姚英民², 李志峰¹, 俞守义¹. 广州地区人类杯状病毒感染的初步研究[J]. 南方医科大学学报, 2004, 24(03): 296-299.

DAI Ying-chun¹, NIE Jun¹, LIU Yi¹, YAO Ying-min², LI Zhi-feng¹, YU Shou-yi¹. Preliminary study of human calicivirus infection in Guangzhou[J]. Journal of Southern Medical University, 2004, 24(03): 296-299.

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