霍奇金淋巴瘤组织中H/RS细胞与其背景细胞的微切割及其IgH基因重排检测

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (03): 286-289.

霍奇金淋巴瘤组织中H/RS细胞与其背景细胞的微切割及其IgH基因重排检测

周新华¹, 赵彤¹, 沈新明¹, 余江², 朱梅刚¹

1. 第一军医大学病理学教研室, 广东, 广州, 510515;
2. 第一军医大学南方医院普外科, 广东, 广州, 510515

出版日期:2004-03-20 发布日期:2004-03-20
基金资助:
收稿日期:2003-9-21。
基金项目:国家自然科学基金(39970692)
作者简介:周新华(1975- ),女,2002年毕业于第一军医大学,硕士,助教,电话:020-61648223,E-mail:balbc@fimmu.com

Detection of IgH gene rearrangement in Reed-Sternberg cells microdissected from classical Hodgkin’s lymphoma

ZHOU Xinhua¹, ZHAO Tong¹, SHEN Xin-ming¹, YU Jiang², ZHU Mei-gang¹

1. 第一军医大学病理学教研室, 广东, 广州, 510515;
2. 第一军医大学南方医院普外科, 广东, 广州, 510515

Online:2004-03-20 Published:2004-03-20

摘要/Abstract

摘要： 目的从基因水平探讨霍奇金淋巴瘤(HL)组织中H/RS细胞(Hodgkin and Reed-Sternberg cells)是否起源于B细胞、H/RS细胞的克隆性以及与背景淋巴细胞的相互关系。方法对33例经典霍奇金淋巴瘤(cHL)石蜡刮片组织进行IgH基因重排分析,并对其中6例重排阳性的病例经B细胞特异性激活蛋白(BSAP)免疫标记,将标记阳性的H/RS细胞和背景淋巴细胞进行微切割后进一步行IgH基因重排分析。结果 16例石蜡刮片组织IgH基因重排阳性。对6例经BSAP标记的cHL成功进行了微切割,其中19管H/RS细胞中,有14管出现重排阳性,细胞数目不同的各管阳性率无显著性差异(P=0.290);12管背景淋巴细胞有2管出现重排阳性,H/RS细胞与背景淋巴细胞的阳性率有显著性差异(P=0.002)。结论支持H/RS细胞来源于B细胞的理论,认为部分背景淋巴细胞可能具有瘤性增生活性,参与H/RS细胞的前体细胞组成。

Abstract: Objective To study the origins and clonality of Hodgkin and Reed-Sternberg (H/RS) cells and their relations with the background lymphocytes in classical Hodgkin’s lymphoma. Method IgH gene rearrangement was detected in paffin-embedded tissues from 33 patients with Hodgkin’s lymphoma and a further analysis of the gene rearrangement was conducted in 6 of the positive cases identified after immunostaining of the sections with B-cell-specific activator protein (BSAP) followed by microdissection of the positivity labeled H/RS cells and background lymphocytes. Results IgH gene rearrangement was identified in 16 of the 33 cases. Microdissection of the lymphoma tissues was successfully performed in the 6 positive cases, and of the 19 tubes of H/RS cells obtained, 14 presented clonal bands of the rearrangement, and difference in cell numbers did not significantly influence the positive rate (P=0.290); in the 12 tubes of microdissected background lymphocytes obtained, 2 were positive for the rearrangement, and the positive rates for the rearrangement significantly differed between H/RS cells and the background lymphocytes (P=0.002). Conclusion The results appear to support the hypothesis that H/RS cells originates from B cells, and a part of the background lymphocytes may possess neoplastic proliferation potentials to function as the precursors of H/RS cells.

中图分类号:

R730.21

周新华¹, 赵彤¹, 沈新明¹, 余江², 朱梅刚¹. 霍奇金淋巴瘤组织中H/RS细胞与其背景细胞的微切割及其IgH基因重排检测[J]. 南方医科大学学报, 2004, 24(03): 286-289.

ZHOU Xinhua¹, ZHAO Tong¹, SHEN Xin-ming¹, YU Jiang², ZHU Mei-gang¹. Detection of IgH gene rearrangement in Reed-Sternberg cells microdissected from classical Hodgkin’s lymphoma[J]. Journal of Southern Medical University, 2004, 24(03): 286-289.

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