双歧杆菌粘附素对脂多糖和H2O2调节肠上皮细胞增殖和凋亡的影响

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (03): 264-268.

双歧杆菌粘附素对脂多糖和H₂O₂调节肠上皮细胞增殖和凋亡的影响

钟世顺, 张振书, 王继德, 赖卓胜, 潘令嘉

第一军医大学南方医院全军消化内科研究所, 广东, 广州, 510515

出版日期:2004-03-20 发布日期:2004-03-20
基金资助:
收稿日期:2003-12-7。
基金项目:广东省自然科学基金资助(010621)
作者简介:钟世顺(1969- ),男,第一军医大学在读博士研究生,主治医师,电话:020-61641544,E-mail:zss@fimmu.com

Effect of Bifidobacterial adhesin on lipopolysaccharide-and H₂O₂-induced proliferation and apoptosis of intestinal epithelial cells in vitro

ZHONG Shi-shun, ZHANG Zhen-shu, WANG Ji-de, LAI Zhuo-sheng, PAN Ling-jia

第一军医大学南方医院全军消化内科研究所, 广东, 广州, 510515

Online:2004-03-20 Published:2004-03-20

摘要/Abstract

摘要： 目的研究双歧杆菌分泌型粘附素对脂多糖(LPS)和H₂O₂体外调节肠上皮细胞增殖和凋亡的影响。方法用³H-TdR掺入法、流式细胞仪和荧光染色等方法观察肠上皮细胞增殖和凋亡的情况。结果 100 μg/L LPS对细胞增殖和凋亡均有促进作用,200 μmol/L H₂O₂可抑制肠上皮细胞增殖,促进凋亡。丫啶橙荧光染色见细胞核染色质致密浓染或裂解成碎片。预先经双歧杆菌分泌型粘附素处理后,LPS组细胞增殖和凋亡率均显著下降,H₂O₂组细胞凋亡明显减少。结论在体外,双歧杆菌分泌型粘附素能抑制LPS和H₂O₂对肠上皮细胞的损害作用,维持肠上皮细胞增殖与凋亡的平衡。

Abstract: Objective To study the effect of Bifidobacterial adhesin on proliferation and apoptosis of intestinal epithelial cell induced by lypopolysaccharide (LPS) and H₂O₂ in vitro. Methods With ³H-TdR incorporation method, flow cytometry and fluorochrome staining, the proliferation and apoptosis of intestinal epithelial cells induced by LPS and H₂O₂ in vitro were studied. Results LPS at the dose of 100 μg/L effectively stimulated the proliferation and apoptosis of cells, whereas H₂O₂ at the dose of 200 μmol/L obviously restrained the proliferative ability while enhanced the apoptosis of the cells. Fluorochrome staining showed cell shrinkage, nuclear condensation and fragmentation under microscope. After treatment with Bifidobacterial adhesin, the cell proliferation and apoptosis decreased significantly in LPS group, and in H₂O₂ group, cell apoptosis was signifi- cantly decreased. Conclusions Bifidobacterial adhesin can protect intestinal epithelial cells from the damage by LPS and H₂O₂, and maintain the balance between the proliferation and apoptosis of the cells.

中图分类号:

R378.2

钟世顺, 张振书, 王继德, 赖卓胜, 潘令嘉. 双歧杆菌粘附素对脂多糖和H₂O₂调节肠上皮细胞增殖和凋亡的影响[J]. 南方医科大学学报, 2004, 24(03): 264-268.

ZHONG Shi-shun, ZHANG Zhen-shu, WANG Ji-de, LAI Zhuo-sheng, PAN Ling-jia. Effect of Bifidobacterial adhesin on lipopolysaccharide-and H₂O₂-induced proliferation and apoptosis of intestinal epithelial cells in vitro[J]. Journal of Southern Medical University, 2004, 24(03): 264-268.

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双歧杆菌粘附素对脂多糖和H₂O₂调节肠上皮细胞增殖和凋亡的影响

Effect of Bifidobacterial adhesin on lipopolysaccharide-and H₂O₂-induced proliferation and apoptosis of intestinal epithelial cells in vitro

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