绿茶多酚抑制晚期糖基化终产物诱导的大鼠血管平滑肌细胞增殖

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (03): 247-251.

绿茶多酚抑制晚期糖基化终产物诱导的大鼠血管平滑肌细胞增殖

欧阳平¹, 彭文烈², 许顶立¹, 赖文岩¹, 徐安龙²

1. 中山大学生命科学院生物化学系, 广东, 广州, 510275;
2. 第一军医大学南方医院心血管内科, 广东, 广州, 510515

出版日期:2004-03-20 发布日期:2004-03-20
基金资助:
基金项目:Supported by the Foundation of Guangzhou Science and Technology Commission.

Green tea polyphenols inhibit advanced glycation end product-induced rat vascular smooth muscle cell proliferation

OUYANG Ping¹, PENG Wen-lie², XU Ding-li¹, LAI Wen-yan¹, XU An-long²

1. 中山大学生命科学院生物化学系, 广东, 广州, 510275;
2. 第一军医大学南方医院心血管内科, 广东, 广州, 510515

Online:2004-03-20 Published:2004-03-20

摘要/Abstract

摘要： 目的本研究观察绿茶多酚对晚期糖基化终产物(AGEs)刺激下离体大鼠胸主动脉血管平滑肌细胞增殖的影响。方法体外培养大鼠主动脉血管平滑肌细胞(VSMCs)应用不同浓度的AGEs分别刺激VSMCs并设立对照组进行比较,将不同剂量的绿茶多酚与AGEs共同作用并设立对照组进行比较,采用非放射性的MTS/PES法确定血管平滑肌细胞的增殖状态。结果与对照组相比,晚期糖基化终产物对血管平滑肌细胞增殖具有明显的刺激作用。在晚期糖基化终产物刺激下,绿茶多酚可明显抑制血管平滑肌细胞的生长。AGEs对p44/42 MAPK磷酸化蛋白表达有显著的增强作用,此作用可被绿茶多酚抑制。结论本研究提示绿茶多酚可抑制晚期糖基化终产物诱导的血管平滑肌细胞增殖及p44/p42 MAPK磷酸化蛋白的表达。

Abstract: Objective To determine the effects of green tea polyphenols(GTP) on advanced glycation end products (AGEs)-induced proliferation and expression of p44/42 mitogen-activated protein kinase (MAPK) of rat vascular smooth muscle cells (VSMCs). Methods Rat aortic VSMCs isolated and cultured in vitro were stimulated with AGEs in the presence or absence of GTP at different concentrations, followed by quantitative analysis of the cell proliferation with colorimetric assay. The p44/42 MAPK activity was evaluated by immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Results Compared with the control cells(without GTP treatment), GTP dose-dependently inhibited AGE-stimulated VSMC proliferation (P<0.05), and the p44/42 MAPK activity was significantly enhanced. The effects of AGEs were antagonized by GTP (372±41 vs 761±56, P<0.05). Conclusion GTP can inhibit the AGE-induced proliferation and p44/42 MAPK expression of rat VSMCs.

欧阳平¹, 彭文烈², 许顶立¹, 赖文岩¹, 徐安龙². 绿茶多酚抑制晚期糖基化终产物诱导的大鼠血管平滑肌细胞增殖[J]. 南方医科大学学报, 2004, 24(03): 247-251.

OUYANG Ping¹, PENG Wen-lie², XU Ding-li¹, LAI Wen-yan¹, XU An-long². Green tea polyphenols inhibit advanced glycation end product-induced rat vascular smooth muscle cell proliferation[J]. Journal of Southern Medical University, 2004, 24(03): 247-251.

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