南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (02): 220-222,225.

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口服霍乱疫苗O139rBS-WC后肠道菌群的改变

吴辉绪1, 陈清1, 李建栋1, 黄秀芬2   

  1. 1. 第一军医大学流行病学教研室, 广东, 广州, 510515;
    2. 广州医学院第一附属医院, 广东, 广州, 510120
  • 出版日期:2004-02-20 发布日期:2004-02-20
  • 基金资助:
    收稿日期:2003-7-6。
    作者简介:吴辉绪(1966-),男,副主任医师,第一军医大学在读硕士研究生,现工作单位为广州军区卫生防疫队,电话:020-87797077,E-mail:hunjeny-wu@mail.china.com
    通讯作者:陈清,教授,电话:020-62648312,E-mail:qingchen@fimmu.com

Dynamics of intestinal flora after oral vaccination with inactivated whole-cell/recombinant B subunit O139 cholera vaccine

WU Hui-xu1, CHEN Qing1, LI Jian-dong1, HUANG Xiu-fen2   

  1. 1. 第一军医大学流行病学教研室, 广东, 广州, 510515;
    2. 广州医学院第一附属医院, 广东, 广州, 510120
  • Online:2004-02-20 Published:2004-02-20

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摘要： 目的 观察国产冻干O139rBS-WC口服霍乱疫苗对健康受试者肠道菌群的影响。方法 利用直接快速涂片法分别于服苗前、服苗后2周末、服苗后3个月末收集受试者的粪便,检查粪便的细菌分布特点。结果 共检查了458份粪便标本,服苗后细菌总数、G+b、G+c、G-c呈下降趋势,而G-b呈显著的增高趋势;口服疫苗后各免疫组之间及与安慰剂组比较,以G+b及G-c变化明显,其中0、14、28 d各服1粒O139rBS-WC 疫苗的免疫组与其他各组间的差异较大;各免疫组在不同采样时间,肠道菌群值有不同的改变,以G-b表现明显。不同性别的受试者在服苗后,G+b、G-b、G-c占总菌群数比值的改变有统计差异。结论 冻干口服O139霍乱rBS-WC疫苗对受试者的肠道菌群有一定的影响。

Abstract: Objective To investigate the change of intestinal flora in subjects receiving oral vaccination with inactivated whole-cell/recombinant B subunit (WC/rBS) O139 cholera vaccine made in China. Methods The fecal smears of 5 groups of subjects receiving the vaccine were collected before and 2 and 3 months after the vaccination to examine the distribution of the intestinal flora. Results In the 458 specimens examined, the total amount of bacteria and percentages of G+b, G+c and G-c were decreased whereas the amount of G-b significantly increased after vaccination. Compared with the placebo control group, the vaccinated groups exhibited significant changes in G+b and G-c, which also varied significantly between the group receiving 3 doses of WC/rBS vaccine and the other vaccinated groups. The vaccination produced distinct changes in the percentage of the intestinal flora at different time points, showing the most obvious effect on G-b. Oral vaccination was shown to affect the percentage of the intestinal flora very likely through its effect on the amount of G-b. The percentages of G+b, G-b and G-c varied significantly between male and female subjects after vaccination. Conclusion Oral vaccination with WC/rBS affects the intestinal flora in human, and the mechanism as well as the implications needs to be further explored.

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