大鼠肝肠缺血再灌注对远隔器官损伤作用的实验研究

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (02): 198-200,203.

大鼠肝肠缺血再灌注对远隔器官损伤作用的实验研究

杨进城¹, 王志伟², 李朝龙¹, 林建华¹, 刘兴国¹, 季锡清¹

1. 第一军医大学南方医院肝胆血管外科, 广东, 广州, 510515;
2. 解放军第157医院普通外科, 广东, 广州, 510510

出版日期:2004-02-20 发布日期:2004-02-20
基金资助:
收稿日期:2003-9-26。
基金项目:广东省计划攻关项目(02B30204)
作者简介:杨进城(1975-),男,第一军医大学在读硕士研究生,电话:020-33161962,E-mail:ycheng75@sohu.com

Multiple organ injury at early stage of intestinal and hepatic ischemia-reperfusion in rats

YANG Jin-cheng¹, WANG Zhi-wei², LI Chao-long¹, LIN Jian-hua¹, LIU Xing-guo¹, JI Qi-xing¹

1. 第一军医大学南方医院肝胆血管外科, 广东, 广州, 510515;
2. 解放军第157医院普通外科, 广东, 广州, 510510

Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要： 目的通过动物实验观察肝肠缺血再灌注对远隔器官的损伤作用。方法健康雄性Wistar大鼠75只,分全肝缺血、肠缺血及肝肠联合缺血3组,分阻断前、阻断末(45 min)、再灌注后0.5、2、6 h共5个时间点,分别活杀5只大鼠取材,测定血浆及组织匀浆中丙二醛和超氧化物歧化酶水平,并观察血尿素氮、血淀粉酶及肌酸激酶等指标的变化。结果肝肠缺血再灌注后,各组血浆及肺、心、肾、胰组织中丙二醛及超氧化物歧化酶水平均发生显著变化,血尿素氮、血淀粉酶及肌酸激酶混合性同工酶水平均呈上升趋势。结论肝肠联合缺血再灌注早期可引起机体其他脏器严重损伤;在相同热缺血时间内,肠缺血可能比肝脏缺血引起更大的其他重要脏器损伤。

Abstract: Objective To investigate the early-stage multiple organ injuries in rats subjected to intestinal and hepatic ischemia-reperfusion. Methods Seventy-five normal male Wistar rats were randomized equally into hepatic ischemia, intestinal ischemia and intestinal-hepatic ischemia groups. Before and at the end of occlusion (45 min), as well as at the time points of 0.5, 2.0 and 6.0 h during the reperfusion, respectively, 5 rats from each group were killed to obtain samples for determination of the contents of malondialdehyde (MDA), superoxide dismutase (SOD) in the blood, lung, kidney, pancreas and heart tissues, along with blood urea nitrogen (BUN), amylase (AMY), and creatine kinase MB (CK-MB). Results The activity of SOD was decreased (P<0.05) and MDA, BUN, AMY and CK-MB levels increased significantly (P<0.05) after ischemia-reperfusion as compared with those before ischemia. Conclusions Intestinal and hepatic ischemia-reperfusion can induce injury of multiple organs at early stage. With the same duration of ischemia-reperfusion, intestinal ischemia may induce severer injury than hepatic ischemia.

中图分类号:

R341.31

杨进城¹, 王志伟², 李朝龙¹, 林建华¹, 刘兴国¹, 季锡清¹. 大鼠肝肠缺血再灌注对远隔器官损伤作用的实验研究[J]. 南方医科大学学报, 2004, 24(02): 198-200,203.

YANG Jin-cheng¹, WANG Zhi-wei², LI Chao-long¹, LIN Jian-hua¹, LIU Xing-guo¹, JI Qi-xing¹. Multiple organ injury at early stage of intestinal and hepatic ischemia-reperfusion in rats[J]. Journal of Southern Medical University, 2004, 24(02): 198-200,203.

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