PDGFR及c-Fos的表达与膀胱癌临床生物学特征的关系

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (02): 177-179,183.

PDGFR及c-Fos的表达与膀胱癌临床生物学特征的关系

姚华强¹, 彭瑛², 钟世镇¹, 何恢绪³, 李忠华¹

1. 第一军医大学临床解剖研究所, 广东, 广州, 510515;
2. 广州军区联勤部东皋干休所, 广东, 广州, 510055;
3. 广州军区总医院泌尿外科, 广东, 广州, 510010

出版日期:2004-02-20 发布日期:2004-02-20
基金资助:
收稿日期:2003-8-9。
基金项目:广东省科技计划项目(A302020401)
作者简介:姚华强(1966-),男,广西北海人,第一军医大学在读博士研究生,主治医师,E-mail:y99333@2911.net

Association of the expressions of platelet-derived growth factor receptor and c-Fos with the biological characteristics of bladder cancer

YAO Hua-qiang¹, PENG Ying², ZHONG Zhi-zhen¹, HE Hui-xu³, LI Zhong-hua¹

1. 第一军医大学临床解剖研究所, 广东, 广州, 510515;
2. 广州军区联勤部东皋干休所, 广东, 广州, 510055;
3. 广州军区总医院泌尿外科, 广东, 广州, 510010

Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要： 目的探讨膀胱移行上皮细胞癌(BTCC)组织中血小板源性生长因子受体(PDGFR)及癌蛋白c-Fos(c-Fos)的表达与BTCC的关系。方法应用免疫组化SP法检测43例BTCC组织、11例正常膀胱组织、14例癌旁组织中PDGFR及c-Fos的表达。结果 PDGFR表达于胞膜、核膜和胞质;c-Fos表达于胞核和胞质。PDGFR和c-Fos在BTCC组织表达阳性率均显著高于正常和癌旁组织(P<0.05);两者在BTCC血管中的染色明显深于正常血管。c-Fos的表达在BTCC的分级中存在显著性差异(P<0.05)。结论 PDGFR和c-Fos的表达可调控膀胱的多种组织,过度表达与BTCC的发生密切相关,并可能与肿瘤的血管形成相关。c-Fos的表达可反映BTCC细胞的增殖状态。

Abstract: Objective To elucidate the relation of platelet-derived growth factor receptor (PDGFR) and c-Fos protein expressions with human bladder transitional epithelial cell carcinoma (BTCC). Methods The expressions of PDGFR and c-Fos were investigated in 11 normal bladder tissue samples, 14 adjacent non-carcinoma tissues and 43 BTCC tissues by means of SP immunohistochemical technique. Results The c-Fos expression was found in the cell nuclei and cytoplasm, and PDGFR in the nuclear membrane, cytoplasm, and cellular membrane. PDGFR and c-Fos were detected in 81.40% and 48.83% of the BTCC tissues respectively, at the rates both significantly higher than those in normal and adjacent non-carcinoma tissues (P<0.05). Correlation between the expression of c-Fos and the tumor grading was noted (P<0.05). The expressions of PDGFR and c-Fos in tumor blood vessels were significantly higher than those in normal vessels. Conclusions The expressions of PDGFR and c-Fos might be involved in the development of BTCC, possibly related to the angiogenesis of the tumors. c-Fos expression can indicated the cell proliferative status of the BTCC.

中图分类号:

R737.14

姚华强¹, 彭瑛², 钟世镇¹, 何恢绪³, 李忠华¹. PDGFR及c-Fos的表达与膀胱癌临床生物学特征的关系[J]. 南方医科大学学报, 2004, 24(02): 177-179,183.

YAO Hua-qiang¹, PENG Ying², ZHONG Zhi-zhen¹, HE Hui-xu³, LI Zhong-hua¹. Association of the expressions of platelet-derived growth factor receptor and c-Fos with the biological characteristics of bladder cancer[J]. Journal of Southern Medical University, 2004, 24(02): 177-179,183.

参考文献

[1] Fudge K, Wang CY, Stearns ME. Immunohistochemistry analysis of platelet-derived growth factor A and B chains and platelet-derived growth factor alpha and beta-receptor expression in benign prostatic hyperplasias and Gleason-graded human prostate adenocarcinomas[J]. Mod Pathol, 1994, 7(3): 549-54.
[2] Cotrota MD, Wang J, Porter PL, et al. Expression ofplatelet-derived growth factor B-chains and platelet-derived growth factor receptor β subunit in human tissue and breast carcinoma[J]. Cancer Res, 1995,55(10): 2703-9.
[3] Puthenveettil JA, Burger MS, Reznikoff CA. Replicative senescence in human uroepitheliai cells[J]. Adv Exp Med Biol, 1999, 462(1):83-91.
[4] Simeonova PP, Wang S, Toriuma W, et al. Arsenic mediates cell proliferation and gene expression in the bladder epithelium:association with activating protein-1 transactivation[J]. Cancer Res,2000, 60(13): 3445-53.
[5] 温进坤,石缨,韩梅,等.原癌基因c-fos和c-jun在血管平滑肌细胞增殖调控中的作用[J].河北医科大学学报,1996,17(1):1-3.Wen JK, Shi Y, Han M, et al. Role of proto-oncogenes c-fos and c-jun in regulation of rat VSMC proliferation[J]. J Hebei Med Univ,1996, 17(1): 1-3.
[6] Kee KH, Lee MJ, Ro JY. Oncoprotein changes in the flat lesions with atypia and invasive neoplasms of the urinary bladder[J]. Oncol Rep, 2001, 8(3): 579-83.
[7] Battergay EJ, Rupp J, Luisa IA, et al. PDGF-BB modulated endothelial and angiogenesis in vitro via PDGF-receptors[J]. J Cell Biol,1994, 125(3): 917-23.
[8] 于士柱,浦佩玉,江德华,等.胶质瘤细胞c-fos和c-myc基因表达与血小板源生长因子B链的纯合二聚体血分泌环活性的观察[J].中华病理学杂志,1999,28(3):182-6.Yu SS, Pu PY, Jian DH, et al. Observations on expression ofc-fos and c-myc genes and activity of PDGF-BB antocrine loop in 67 human gliomas[J]. Chin J Pathol, 1999, 28(3): 182-6.
[9] Whiteside C, Munk S, Zhou X, et al. Chelation of intracellular calcium prevents mesangial cell proliferative responsiveness[J]. J Am Soc Nephrol, 1998, 9(1): 14-25.