重组海葵溶细胞素及平颏海蛇磷脂酶A2对血管外膜成纤维细胞增殖的影响

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (02): 158-160.

重组海葵溶细胞素及平颏海蛇磷脂酶A₂对血管外膜成纤维细胞增殖的影响

欧阳平¹, 姜孝玉², 杨文利², 赖文岩¹, 徐安龙²

1. 第一军医大学南方医院心血管内科, 广东, 广州, 510515;
2. 中山大学生命科学学院国家高技术计划海洋生物功能基因组开放实验室, 广东, 广州, 510275

出版日期:2004-02-20 发布日期:2004-02-20
基金资助:
收稿日期:2003-10-25。
基金项目:国家863计划项目(2001AA628090);广州市科委科技攻关引导项目(2002z3-C7111)
作者简介:欧阳平(1966-),男,医学博士,生物学博士后,副教授,电话:020-61647640,E-mail:ypou@fimmu.com

Effects of recombinant Sagartia rosea cytolysin and Lapemis hardwickii phospholipase A₂ on adventitial fibroblasts proliferation

OUYANG Ping¹, JIANG Xiao-yu², YANG Wen-li², LAI Wen-yan¹, XU An-long²

1. 第一军医大学南方医院心血管内科, 广东, 广州, 510515;
2. 中山大学生命科学学院国家高技术计划海洋生物功能基因组开放实验室, 广东, 广州, 510275

Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要： 目的观察重组海葵溶细胞素(rSrc)及平颏海蛇磷脂酶A₂(rPLA₂)对成纤维细胞增殖的影响。方法体外培养成纤维细胞,应用不同浓度的rSrc和rPLA₂分别进行作用并设立对照进行比较,采用非放射性的MTS/PES法确定成纤维细胞的增殖状态。结果各组细胞增殖率分别为:对照组0.840±0.061、rSrc 100 μg/ml组0.263±0.044、rSrc 10 μg/ml组0.418±0.054、rSrc 1 μg/ml组0.605±0.063、rSrc 100 ng/ml组0.772±0.054、rSrc 10 ng/ml组0.906±0.072、rPLA₂ 100 μg/ml组0.498±0.076、rPLA₂ 10 μg/ml组0.937±0.112、rPLA₂ 1 μg/ml组0.978±0.145。统计分析显示,低至1 μg/ml的rSrc仍能明显抑制大鼠成纤维细胞的增殖(P<0.01),并呈现剂量依赖性(P<0.05)。rPLA₂ 100 μg/ml组与对照组相比细胞增殖率有显著差异(P<0.05);rPLA₂ 10 μg/ml和rPLA₂ 1 μg/ml组与对照组相比均无显著差异(P>0.05)。结论 rSrc和rPLA₂分别对血管外膜成纤维细胞增殖有一定的抑制作用。

Abstract: Objective To observe the effects of recombinant Sagartia rosea cytolysin (rSrc) and Lapemis hardwickii phospholipase A₂(rPLA₂) on adventitial fibroblasts proliferation. Methods NIH-3T3 cells were cultured and treated with rSrc and rPLA₂ at different concentrations for observation of cell proliferation using non-radioactive MTS/PES assay in comparison with the control group. Results The ratio of cell proliferation was 0.840±0.061 in the control group, and was 0.263±0.044, 0.418±0.054, 0.605±0.063, 0.772±0.054 and 0.906±0.072 in rSrc groups corresponding to rSrc concentrations of 100, 10, 1 μg/ml and 100, 10 ng/ml respectively. rSrc was found to significantly inhibit fibroblast proliferation in a dose-dependent manner when the concentration used was above 1 μg/ml (P<0.05), as compared with the control group (P<0.05). The ratio of cell proliferation was 0.498±0.076, 0.937±0.112 and 0.978±0.145 in rPLA₂ groups corresponding to rPLA₂ concentrations of 100, 10, 1 μg/ml respectively, indicating that rPLA₂ also significantly inhibited fibroblast proliferation at the concentration of 100 μg/ml (P<0.05). Conclusion rSrc and rPLA₂ can both significantly inhibit adventitial fibroblast proliferation.

中图分类号:

R329.2

欧阳平¹, 姜孝玉², 杨文利², 赖文岩¹, 徐安龙². 重组海葵溶细胞素及平颏海蛇磷脂酶A₂对血管外膜成纤维细胞增殖的影响[J]. 南方医科大学学报, 2004, 24(02): 158-160.

OUYANG Ping¹, JIANG Xiao-yu², YANG Wen-li², LAI Wen-yan¹, XU An-long². Effects of recombinant Sagartia rosea cytolysin and Lapemis hardwickii phospholipase A₂ on adventitial fibroblasts proliferation[J]. Journal of Southern Medical University, 2004, 24(02): 158-160.

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重组海葵溶细胞素及平颏海蛇磷脂酶A₂对血管外膜成纤维细胞增殖的影响

Effects of recombinant Sagartia rosea cytolysin and Lapemis hardwickii phospholipase A₂ on adventitial fibroblasts proliferation

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