Plunc序列启动子转基因小鼠模型的建立

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (02): 155-157.

Plunc序列启动子转基因小鼠模型的建立

杨玉芳, 丁彦青, 张玲, 梁莉

第一军医大学肿瘤研究所, 广东, 广州, 510515

出版日期:2004-02-20 发布日期:2004-02-20
基金资助:
收稿日期:2003-9-8。
基金项目:国家“863”自然科学基金(2001AA₂16101)
作者简介:杨玉芳(1964-),女,甘肃兰州人,第一军医大学在读博士研究生,副主任医师,电话:020-61648114-89100,E-mail:yyf@fimmu.com

Establishment of transgenic mouse model carring the promoter of plunc sequence

YANG Yu-fang, DING Yan-qing, ZHANG Ling, LIANG Li

第一军医大学肿瘤研究所, 广东, 广州, 510515

Online:2004-02-20 Published:2004-02-20

摘要/Abstract

摘要： 目的利用携带有鼻咽组织相对特异性启动子plunc构建的载体建立转基因动物模型。方法将plunc-EGFP质粒用XhoⅠ酶切线性化,胶回收线性化片段,稀释浓度4 μg/ml。采用显微注射法将外源基因注射入小鼠受精卵细胞内。结果产13只小鼠。PCR检测基因整合,其中阳性12只,阳性率92.30%;Southern blot检测阳性3只,阳性率23%。结论鼻咽组织相对特异性启动子plunc可有效地将外源基因整合入小鼠体内。

Abstract: Objective To construct the vector using specific nasopharynx tissue promoter plunc to establish transgeneic mouse model. Methods Plunc-EGFP plasmid was digested with XhoⅠ, the purified linearized DNA fragments were recovered by gel extraction and diluted to the final concentration of 4 μg/ml, before introduced into fertilized one-cell mouse eggs by pronuclear microinjection. Results Thirteen founder mice were obtained, 12 of which were positive for the integrated EGFP gene as detected by PCR, and 3 were positive shown by Southern blotting. Conclusion Specific nasopharynx tissue promoter plunc can effectively induce exogenous gene integration into the mouse genome.

中图分类号:

R392-33

杨玉芳, 丁彦青, 张玲, 梁莉. Plunc序列启动子转基因小鼠模型的建立[J]. 南方医科大学学报, 2004, 24(02): 155-157.

YANG Yu-fang, DING Yan-qing, ZHANG Ling, LIANG Li. Establishment of transgenic mouse model carring the promoter of plunc sequence[J]. Journal of Southern Medical University, 2004, 24(02): 155-157.

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