SOD与NaHCO3对重症失血性休克低血管反应性的影响

摘要/Abstract

摘要： 目的探讨超氧化物岐化酶(SOD)和NaHCO₃治疗重症失血性休克低血管反应性的效果。方法将28只重症失血性休克SD大鼠随机均分为SOD组、NaHCO₃组、SOD+ NaHCO₃ 3个治疗组以及1个对照组,分别观察用药后各组大鼠的血管反应性、血压、微动脉血流量和24 h存活率。结果休克2 h后,各组动物微血管对去甲肾上腺素(NE)的反应性显著减低,NE阈值比失血前提高24~27倍。复苏治疗2 h后,3个治疗组血管反应性都有回升,其中以SOD + NaHCO₃组效果最显著,NE阈值下降至失血前的4.7倍。此外,给SOD+ NaHCO₃后再给多巴胺的升压效应最为显著,是对照组的1.9倍,且在回输血液后动物血压稳定在13.33 kPa以上;治疗2 h后,微动脉血流量是对照组的2.54倍,动物存活时间比对照组延长2.9倍。结论 SOD和NaHCO₃对重症失血性休克大鼠的血管低反应性有恢复作用;先给SOD+NaHCO₃再给升压药物(多巴胺)可明显稳定提升动物血压,并提高动物存活率,提示SOD+碱可能是治疗重症失血性休克低血管反应性的一种新的治疗方法。

Abstract: Objective To investigate the effect of SOD and NaHCO₃ on the vascular hyporeactivity after severe hemorrhagic shock in rats. Methods Twenty eight Sprague-Dawley rats were divided randomly into 4 groups of 7 each: SOD-treated group, NaHCO₃-treated group, SOD+NaHCO₃-treated group,and normal saline-treated control group. The responses of arterioles to norepinephrine (NE), the effects of dopamine on blood pressure, blood flow velocity in arterioles, the mean arterial pressure (MAP), and the survival time within 24 h were measured. Results Two hours after shock, the arteriolar vasoreactivity was significantly reduced and threshold concentration of NE was increased by 24 to 27 folds. After beting treated separately with SOD, NaHCO₃ and SOD+NaHCO₃, arteriolar vasoreactivity was restored to some extent, among which the SOD+NaHCO₃ group obtained a most significant effect with decreased threshold concentration of NE to 21% of its normal level. In SOD+NaHCO₃-treated group, injection of dopamine indicated a most significant effect on MAP with 1.9 times more than that in control group, and the enhanced MAP was maintained at a level of more than 13.33 kPa after reinfusion of shed blood. The blood flow in arteriole was 2.54-fold higher than that in control group after 2 h resuscitation. The average survival time in SOD+NaHCO₃-treated group was 2.9 times longer than that in control group. Conclusions SOD and NaHCO₃ could both recover the lower vasoreactivity and increase the enhancing effect of dopamine on blood pressure of rats in severe hemorrhagic shock, suggesting that coadministration of SOD and NaHCO₃ could be a new approach to the treatment of severe hemorrhagic shock.

中图分类号:

金建秋, 赵克森, 赵桂玲, 黄绪亮, 潘秉兴. SOD与NaHCO₃对重症失血性休克低血管反应性的影响[J]. 南方医科大学学报, 2004, 24(02): 144-147.

JIN Jian-qiu, ZHAO Ke-sen, ZHAO Gui-ling, HUANG Xu-liang, PAN Bing-xing. Effect of SOD and NaHCO₃ on the vascular hyporeactivity of rats after severe hemorrhagic shock[J]. Journal of Southern Medical University, 2004, 24(02): 144-147.

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SOD与NaHCO₃对重症失血性休克低血管反应性的影响

Effect of SOD and NaHCO₃ on the vascular hyporeactivity of rats after severe hemorrhagic shock

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