RT-PCR法检测肾癌组织中MN/CAⅨ基因表达

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (01): 99-101.

RT-PCR法检测肾癌组织中MN/CAⅨ基因表达

姜耀东¹, 郑少斌¹, 王战会²

1. 第一军医大学南方医院, 泌尿外科, 广东, 广州, 510515;
2. 第一军医大学南方医院, 感染内科实验室, 广东, 广州, 510515

出版日期:2004-01-20 发布日期:2004-01-20
基金资助:
收稿日期:2003-9-27。
作者简介:姜耀东(1974- ),男,1997年毕业于第一军医大学,现为在读硕士研究生,医师,电话:020-61647097,E-mail:jyd@fimmu.com

Detection of carbonic anhydrase Ⅸ gene expression in renal cell carcinoma with reverse transcription-PCR

JIANG Yao-dong¹, ZHENG Shao-bin¹, WANG Zhan-hui²

1. 第一军医大学南方医院, 泌尿外科, 广东, 广州, 510515;
2. 第一军医大学南方医院, 感染内科实验室, 广东, 广州, 510515

Online:2004-01-20 Published:2004-01-20

摘要/Abstract

摘要： 目的评价RT-PCR法检测碳酸酐酶Ⅸ(MN/CAⅨ)基因在肾癌组织的表达的可靠性。方法以5例肾癌和5例正常肾组织为研究对象,提取总RNA,两步法进行RT-PCR反应,用MN/CAⅨ的特异性引物扩增,2%琼脂糖凝胶电泳后观察并测序。结果经过测序证实5例肾癌组织的RT-PCR产物中有4例可见特异的MN/CAⅨ条带,而正常肾组织的RT-PCR产物均无相应的MN/CAⅨ条带。结论 RT-PCR法检测MN/CAⅨ在肾癌组织中的表达是准确、可靠的。

Abstract: Objective To evaluate the reliability of reverse transcription (RT) -PCR in detecting the expression of the carbonic anhydrase Ⅸ(MN/CAⅨ) gene in renal cell carcinoma (RCC).Methods MN/CAⅨwas examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAⅨwas designed and two-step RT-PCR of the ex-tracted total RNA performed. The products was examined by 2% agrose electrophoresis and identified by sequence nanalysis.Results Specific bands of MN/CAⅨwere found in RT-PCR products of 4 case of RC C tissues, whereas could not be seen in normal renal tissues.Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.

中图分类号:

R730.53

姜耀东¹, 郑少斌¹, 王战会². RT-PCR法检测肾癌组织中MN/CAⅨ基因表达[J]. 南方医科大学学报, 2004, 24(01): 99-101.

JIANG Yao-dong¹, ZHENG Shao-bin¹, WANG Zhan-hui². Detection of carbonic anhydrase Ⅸ gene expression in renal cell carcinoma with reverse transcription-PCR[J]. Journal of Southern Medical University, 2004, 24(01): 99-101.

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