大肠侧向发育型肿瘤原代培养细胞的电镜观察

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (01): 79-80,84.

大肠侧向发育型肿瘤原代培养细胞的电镜观察

韩宇晶¹, 赖卓胜¹, 王亚东¹, 姜泊¹, 周志涛², 周殿元¹

1. 第一军医大学, 南方医院全军消化内科研究所, 广东, 广州, 510515;
2. 第一军医大学电镜室, 广东, 广州, 510515

出版日期:2004-01-20 发布日期:2004-01-20
基金资助:
收稿日期:2003-4-16。
基金项目:广东省基金项目子课题(2002c30307)
作者简介:韩宇晶(1970- ),女,第一军医大学在读硕士研究生,主治医师,电话:020-61641888-87100
通讯作者:姜泊,主任医师,博士生导师,电话:020-61641541

Electron microscopic observation of primary cultured laterally spreading tum or cell line

HAN Yu-jing¹, LAI Zhuo-sheng¹, WANG Ya-dong¹, JIANG Bo¹, ZHOU Zhi-tao², ZHOU Dian-yuan¹

1. 第一军医大学, 南方医院全军消化内科研究所, 广东, 广州, 510515;
2. 第一军医大学电镜室, 广东, 广州, 510515

Online:2004-01-20 Published:2004-01-20

摘要/Abstract

摘要： 目的研究大肠侧向发育型肿瘤(LST)细胞经原代培养的细胞株的超微结构特点。方法将1例经肠镜切除的直肠LST细胞进行原代培养建株,然后进行扫描电镜及透射电镜观察,并与直肠腺癌及正常粘膜上皮细胞超微结构进行对照。结果 LST原代培养的细胞表面被覆大量微绒毛,胞浆内有较多溶酶体、线粒体和吞噬颗粒,核异型性明显,核仁巨大,异型核沟。结论 LST原代培养细胞具有高度恶性倾向。

Abstract: Objec tive To observe the microscopic characteristics of laterally spreading tumor (LST) cell line in primary cul-ture.Methods The cells isolated from a rectum LST s pecimen obtained by endoscopic mucosal resection was primary cul-tured,followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocar-cinoma and normal mucosa of the rectum.Results Scan ning and transmission electron microscopes both revealed numerous microvilli co vering the surface of the LST cells,and the cytoplasm contained large quantity of lysosomes,mitochondria and phagosomes.Obviously heterogeneous cell nuclei were present with abnormal nuclear fossa and huge nucleoli.Conclusion The cultu red LST cells are highly malignant.

中图分类号:

R735.34

韩宇晶¹, 赖卓胜¹, 王亚东¹, 姜泊¹, 周志涛², 周殿元¹. 大肠侧向发育型肿瘤原代培养细胞的电镜观察[J]. 南方医科大学学报, 2004, 24(01): 79-80,84.

HAN Yu-jing¹, LAI Zhuo-sheng¹, WANG Ya-dong¹, JIANG Bo¹, ZHOU Zhi-tao², ZHOU Dian-yuan¹. Electron microscopic observation of primary cultured laterally spreading tum or cell line[J]. Journal of Southern Medical University, 2004, 24(01): 79-80,84.

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