rhIL-10对肿瘤坏死因子-α诱导的血管外膜成纤维细胞增殖的影响

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (01): 50-52.

rhIL-10对肿瘤坏死因子-α诱导的血管外膜成纤维细胞增殖的影响

欧阳平¹, 孟素荣¹, 刘彦波¹, 许顶立¹, 赖文岩¹, 彭立胜², 徐安龙²

1. 广州第一军医大学附属南方医院心血管内科, 广东, 广州, 510515;
2. 广州中山大学生命科学学院生物化学系, 广东, 广州, 510275

出版日期:2004-01-20 发布日期:2004-01-20
基金资助:
收稿日期:2003-12-8。
基金项目:广州市科委科技攻关项目(JB00000448165)
作者简介:wenli@fimmu.com

Effects of recombinant human interleukin-10on tumor necorsis factor-α-induced adventitial fibroblast proliferationIn vitro

OUYANG Ping¹, MENG Su-rong¹, LIU Yan-bo¹, XU Ding-li¹, LAI Wen-yan¹, PENG Li-sheng², XU An-long²

1. 广州第一军医大学附属南方医院心血管内科, 广东, 广州, 510515;
2. 广州中山大学生命科学学院生物化学系, 广东, 广州, 510275

Online:2004-01-20 Published:2004-01-20

摘要/Abstract

摘要： 目的观察重组人白细胞介素-10(rhIL-10)对肿瘤坏死因子-α(TNF-α)诱导的成纤维细胞增殖的影响。方法体外培养NIH/3T3细胞,应用rhIL-10与TNF-α共同作用并设立对照进行比较,采用MTS/PES法确定NIH/3T3细胞的增殖状态。应用流式细胞仪测定细胞周期。结果 TNF-α对NIH/3T3细胞增殖具有明显的刺激作用。rhIL-10单独应用对NIH/3T3细胞生长没有影响。在TNF-α刺激下,一定剂量的rhIL-10可抑制NIH/3T3细胞的增殖。rhIL-10可使TNF-α作用下的NIH/3T3细胞大部分处于G₀/G₁期。结论 rhIL-10能明显抑制由TNF-α刺激的成纤维细胞增殖。

Abstract: Objective To observe the effects of recombin an t human interleukin-10 (rhIL-10) on adventitial fibroblast prolifer-ation induce d by tumor necrosis factor-α(TNF-α)in vitro.Methods In this controlled stud y, NIH/3T3 cells cultured In vitrowere treated with TNF-αand recombinant human interleukin-10 (rhIL-10), respectively, and the cell proliferation was deter-mi ned by non-radioactive MTS/PES assay. Cell cycle analysis was performed using fl ow cytomertry.Results Compared with the control cells, TNF-αsignificantly sti mulated NIH/3T3 cell proliferation, wherea. rhIL-10 had no such effect. With TNF -αstimulation, rhIL-10 at the dose as low as 1 ng/ml inhibited the proliferatio n of NIH/3T3 cells (P<0.05). The number of cells in G₀/G₁ phase treated with both TNF-αand rhIL-10 was higher than those treated wit h TNF-αalone (P<0.05), as shown by flow cytometry.Conclusion rhIL-10 can sign ificantly inhibit the proliferation of adventitial fibroblasts induced by TNF-αIn vitro.

中图分类号:

R541

欧阳平¹, 孟素荣¹, 刘彦波¹, 许顶立¹, 赖文岩¹, 彭立胜², 徐安龙². rhIL-10对肿瘤坏死因子-α诱导的血管外膜成纤维细胞增殖的影响[J]. 南方医科大学学报, 2004, 24(01): 50-52.

OUYANG Ping¹, MENG Su-rong¹, LIU Yan-bo¹, XU Ding-li¹, LAI Wen-yan¹, PENG Li-sheng², XU An-long². Effects of recombinant human interleukin-10on tumor necorsis factor-α-induced adventitial fibroblast proliferationIn vitro[J]. Journal of Southern Medical University, 2004, 24(01): 50-52.

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