南方医科大学学报

南方医科大学学报 ›› 2025, Vol. 45 ›› Issue (10): 2118-2125.doi: 10.12122/j.issn.1673-4254.2025.10.08

• • 上一篇    

LINC00261通过靶向miR-23a-3p/ZNF292轴抑制食管鳞状细胞癌的增殖、侵袭与转移

米源1(), 李旭哲2, 王占鹏3, 刘延杰4, 宋春涛1, 王澜涛1, 王雷4()   

  1. 1.河北医科大学第四医院急诊科，河北 石家庄 050000
    2.河北医科大学基础医学院临床医学系，河北 石家庄 050000
    3.故城县医院急诊科，河北 衡水 053000
    4.河北医科大学第四医院胸二科，河北 石家庄 050000
  • 收稿日期:2025-05-12 出版日期:2025-10-20 发布日期:2025-10-24
  • 通讯作者: 王雷 E-mail:miyuan_medicine@163.com;wangleidoctor@ hebmu.edu.cn
  • 作者简介:米 源，博士，主治医师，E-mail: miyuan_medicine@163.com
  • 基金资助:
    河北省医学科学研究课题计划资助(20240625);河北省省级科技计划项目(22377769D);河北省省级科技计划基础研究专项（自然科学基金）联合基金项目(H202206391);河北医科大学第四医院科研创新团队项目(2023B02);中国青年医学创新研究科研课题项目(P250125108786);中国抗癌协会肿瘤领域区域性科研培育基金项目(CESTDQLCORP300-07)

LINC00261 suppresses esophageal squamous cell carcinoma proliferation, invasion, and metastasis by targeting the miR-23a-3p/ZNF292 axis

Yuan MI1(), Xuzhe LI2, Zhanpeng WANG3, Yanjie LIU4, Chuntao SONG1, Lantao WANG1, Lei WANG4()   

  1. 1.Department of Emergency Medicine, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China
    2.Department of Clinical Medicine, School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050000, China
    3.Department of Emergency Medicine, Gucheng County Hospital, Hengshui 053000, China
    4.Department of Thoracic Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China
  • Received:2025-05-12 Online:2025-10-20 Published:2025-10-24
  • Contact: Lei WANG E-mail:miyuan_medicine@163.com;wangleidoctor@ hebmu.edu.cn

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摘要：

目的 探讨长链非编码RNA LINC00261对食管鳞状细胞的增殖、侵袭及转移等生物学行为的影响。 方法 通过GEO数据库中GSE149612数据集分析食管鳞状细胞癌中的差异分子。收集临床食管鳞状细胞癌及正常食管组织样本及多种食管鳞状细胞癌细胞系细胞和正常食管上皮细胞HEEC细胞，通过PCR技术检测LINC00261的表达变化。过表达LINC00261再分为pcDNA组和pcDNA-LINC00261组，CCK-8、克隆形成实验检测细胞增殖能力，Transwell和流式细胞术分析其对细胞侵袭转移和凋亡的影响。ENCORI等相关生物信息学网站对LINC00261的靶点进行预测，双荧光素酶实验及Western blotting实验进行验证，裸鼠皮下移植瘤实验验证了LINC00261对食管鳞状细胞癌的影响。 结果 LINC00261在食管鳞状细胞癌中显著低表达，并且在临床食管鳞状细胞癌样本及细胞系中同样表达量明显降低（P<0.05）；通过过表达LINC00261，发现其明显抑制了食管鳞状细胞癌的增殖、侵袭和转移并促进了肿瘤细胞的凋亡（P<0.05）。通过生物信息学网站的预测和双荧光素酶报告基因测定，验证了LINC00261靶向miR-23a-3p/ZNF292的关系。裸鼠皮下移植瘤实验发现通过过表达LINC00261可以明显抑制食管鳞状细胞癌的增殖和转移（P<0.0001）。 结论 LINC00261可以通过靶向miR-23a-3p/ZNF292抑制人食管鳞状细胞癌的增殖、侵袭和转移，为食管鳞状细胞癌的治疗提供新的依据。

关键词: 食管鳞状细胞癌, LINC00261, miR-23a-3p, ZNF292

Abstract:

Objective To evaluate the regulatory effects of lncRNA LINC00261 on proliferation, invasion, and metastasis of esophageal squamous cell carcinoma (ESCC) cells. Methods The differentially expressed RNAs in ESCC were identified using the GSE149612 dataset from the GEO database. PCR was used to detect LINC00261 expression levels in clinical ESCC and normal esophageal tissue samples and in multiple ESCC cell lines and normal esophageal epithelial cells (HEEC). In ESCC cells, the effects of overexpression of LINC00261 on cell proliferation, invasion, metastasis and apoptosis were analyzed using CCK-8 assay, clone formation assay, Transwell assay and flow cytometry. The potential targets of LINC00261 were predicted using bioinformatics tools including ENCORI and verified using dual-luciferase reporter assay and Western blotting. The effects of LINC00261 overexpression on ESCC were confirmed in a nude mouse model bearing ESCC xenograft. Results Analysis of the GSE149612 dataset revealed significantly lower LINC00261 expression in ESCC tissues and cell lines. In cultured ESCC cells, LINC00261 overexpression markedly suppressed cell proliferation, invasion, and metastasis and promoted cell apoptosis. Dual-luciferase reporter assays confirmed that LINC00261 targets the miR-23a-3p/ZNF292 axis. In the tumor-bearing mouse model, LINC00261 overexpression significantly inhibited ESCC xenograft proliferation and metastasis. Conclusion LINC00261 suppresses ESCC progression by targeting the miR-23a-3p/ZNF292 axis, suggesting a potential therapeutic strategy for ESCC treatment.

Key words: esophageal squamous cell carcinoma, LINC00261, miR-23a-3p, ZNF292