南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (12): 2061-2070.doi: 10.12122/j.issn.1673-4254.2023.12.10

• • 上一篇    下一篇

纳米脂肪联合富血小板血浆治疗大鼠压力性损伤创面的实验研究

张 铭，于 浩，邵 阳，宋国栋，陈铭锐，刘顺利   

  1. 解放军第九六〇医院烧伤整形科，山东 济南 250031；济南市中心医院烧伤整复外科，山东 济南 250013；潍坊医学院临床医学院，山东 潍坊 261053
  • 出版日期:2023-12-20 发布日期:2023-12-29

Effect of nanofat combined with platelet-rich plasma for treatment of pressure injury wounds in rats

ZHANG Ming, YU Hao, SHAO Yang, SONG Guodong, CHEN Mingrui, LIU Shunli   

  1. Department of Burn and Plastic Surgery, 960th Hospital of PLA, Jinan 250031, China; Department of Burn and Plastic Surgery, Jinan Central Hospital, Jinan 250013, China; Clinical Medical College of Weifang Medical College, Weifang 261053, China
  • Online:2023-12-20 Published:2023-12-29

RichHTML

8

PDF

180

摘要： 目的 探讨同种异体纳米脂肪联合富血小板血浆对大鼠压力性损伤创面的影响及治疗效果。方法 将40只SD大鼠成功建立压力性损伤模型后，随机分为4组（10只/组）：纳米脂肪联合富血小板血浆处理组（NP组）、单纯纳米脂肪处理组（N组）、单纯富血小板血浆处理组（P组）、对照组（C组）。处理后观察创面愈合情况，第1、3、5、7、10、14天拍照，计算创面愈合率；第5、10、14天取创面标本行HE染色，Masson染色，CD31、MCP-1免疫组织化学染色及VEGF蛋白质免疫印迹检测，观察创面炎性细胞浸润，胶原生成、沉积及排列，血管新生、炎性因子表达及VEGF表达情况。结果 压力性损伤模型及纳米脂肪制备成功，PRP内血小板计数约为正常全血的6.2倍。创面表面观察显示，NP组愈合最快，其各观察点的创面愈合率明显高于其余3组（P<0.05），N组与P组无明显差异（P>0.05），第7、14天时明显高于C组（P<0.05）。HE染色显示，NP组第5天炎性细胞浸润增强，真皮及表皮生长快于其他组，第14天基本完全愈合，N组与P组各时间点组织变化相似，且优于C组（P<0.05）。Masson染色显示，NP组各时间点压力性损伤创面相对胶原表达量以及排列顺序明显优于其他组（P<0.05），N组及P组在各时间点也较C组有明显差异（P<0.05）。CD31免疫组化显示不同时间点NP组血管新生数目高于其余3组（P<0.05），N组及P组各时间点差异无统计学意义（P>0.05），但两组新生血管数目均高于C组。MCP-1免疫组化结果示，第5天NP组压力性损伤创面MCP-1相对表达强于其他组（P<0.05），N组及P组同时亦高于C组（P<0.05）。VEGF蛋白质免疫印迹检测显示，NP组PI创面3个时间点VEGF表达明显高于其余3组（P<0.05），第5、10天时N组、P组及C组相近，仅在第14天时N组及P组明显强于C组（P<0.05）。结论 纳米脂肪联合富血小板血浆治疗压力性损伤创面可显著促进创面愈合，两者联合具有协同作用，为压力性损伤创面的临床治疗提供一种新的可靠方案。

关键词: 压力性损伤；纳米脂肪；富血小板血浆；创面愈合

Abstract: Objective To investigate the effect of allogeneic nanofat combined with platelet-rich plasma (PRP) for treatment of pressure injury wounds in rats. Methods Forty SD rat models with pressure injury wounds were randomly divided into 4 groups (n=10) for treatment with nanofat, PRP (platelet count about 6.2 times that of normal whole blood), nanofat combined with PRP, or PBS only (control group).Wound healing was observed on days 1, 3, 5, 7, 10 and 14 and wound healing rate was calculated. On days 5, 10 and 14, tissue samples were taken from the wounds for HE staining, Masson staining, immunohistochemistry, and Western blotting to observe inflammatory cell infiltration, collagen formation, deposition and arrangement, angiogenesis, inflammatory factor expression and VEGF expression in the wounds. Results The combined treatment with nanofat and PRP achieved the highest wound healing rates at all the time points of observation (P<0.05), enhanced inflammatory cell infiltration on day 5, and accelerated dermal and epidermal growth compared with the other treatments (P<0.05). The combined treatment also more effectively promoted collagen expression and its regular arrangement (P<0.05) and enhanced angiogenesis in the wounds than nanofat and PRP alone, without significant difference between the latter two treatments (P>0.05). Immunohistochemistry and Western blotting showed that the expressions of MCP-1 and VEGF in the wounds were the highest in the combined treatment group (P<0.05) and were higher in nanofat and PRP treatment groups than in the control group (P<0.05). Conclusion Nanofat combined with PRP can significantly promote healing of pressure injury wounds in rats, and their synergistic effect sheds light on a new strategy for treatment of pressure injury wounds.

Key words: pressure injury; nanofat; platelet rich plasma; wound healing