南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (11): 1984-1988.doi: 10.12122/j.issn.1673-4254.2023.11.21

• • 上一篇    

尿路上皮癌抗原1促进滋养细胞功能及对上皮-间质转化转化的调控作用

孙建华，张 逸，杨丽萍，周 立，卢喜洋，李久现，陈 萍   

  1. 河南中医药大学第一临床医学院，河南 郑州 450046；河南中医药大学第一附属医院妇产科，河南 郑州 450099
  • 出版日期:2023-11-20 发布日期:2023-12-08

High expression of long noncoding RNA UCA1 promotes invasion, migration and epithelial-mesenchymal transition of trophoblasts in vitro

SUN Jianhua, ZHANG Yi, YANG Liping, ZHOU Li, LU Xiyang, LI Jiuxian, CHEN Ping   

  1. First Clinical College of Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China; Department of Obstetrics and Gynecology, First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450099, China
  • Online:2023-11-20 Published:2023-12-08

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摘要： 目的 研究尿路上皮癌抗原1（UCA1）对滋养细胞（HTR-8/SVneo）侵袭和迁移能力及上皮-间质转化（EMT）特性的影响，探索UCA1在输卵管妊娠（TP）发生发展过程中的作用及机制。方法 实验设为空白对照组及IL-6刺激组，采用qRT-PCR检测滋养细胞UCA1表达，划痕实验检测细胞迁移功能；通过瞬时转染构建HTR-8/SVneo细胞UCA1沉默细胞模型，细胞设为空白对照组及UCA1沉默组，分别采用划痕实验和transwell侵袭实验、qRT-PCR及Western blot实验检测UCA1对HTR-8/SVneo细胞迁移、侵袭能力及EMT标志物表达水平的影响。结果 IL-6干预后HTR-8/SVneo细胞迁移距离增加，UCA1表达显著上调（P<0.01）；沉默UCA1后HTR-8/SVneo细胞UCA1表达明显下调，迁移距离明显缩短、穿膜细胞个数明显减少（P<0.01），EMT上皮标志物E-cadherin mRNA表达显著上调（P<0.01），间质标志物integrin β3、Vimentin、N-cadherin mRNA表达下调（P<0.01、P<0.05、P<0.01）；EMT上皮标志物E-cadherin蛋白表达上调（P<0.01），间质标志物integrin β3、Vimentin、N-cadherin蛋白表达下调（P<0.01）。结论 UCA1可能是促进TP发生发展的关键基因，有望成为该疾病新的治疗靶点。

关键词: 输卵管妊娠；UCA1；迁移侵袭；上皮-间质转化

Abstract: Objective To investigate the role of urothelial carcinoma antigen 1 (UCA1) in regulation of invasion, migration and epithelial-mesenchymal transition (EMT) of trophoblast HTR-8/SVneo cells and its association with tubal pregnancy. Methods Cultured HTR- 8/SVneo cells stimulated with interleukin-6 (IL-6) were examined for changes in UCA1 expression and cell migration ability using qRT-PCR and scratch assay, respectively. A HTR-8/SVneo cell model with UCA1 silencing was constructed by transient transfection, and the migration and invasion abilities of the cells were assessed using Scratch assay and Transwell assay; qRT-PCR and Western blotting were performed to detect the mRNA and protein expression levels of EMT markers. Results HTR-8/SVneo cells stimulated with IL-6 exhibited significantly increased migration ability and up-regulated expression of UCA1 (P<0.01). UCA1 silencing obviously suppressed migration and invasion abilities of HTR-8/SVneo cells (P<0.01), significantly up-regulated the mRNA and protein expressions of EMT epithelial marker E-cadherin (P<0.01), and down-regulated the expressions of the mesenchymal markers integrin β3, vimentin and N-cadherin (P<0.05). Conclusion UCA1 may be a key gene that promotes the occurrence of tubal pregnancy and thus provides a new therapeutic target for tubal pregnancy.

Key words: tubal pregnancy; urothelial carcinoma antigen 1; migration; invasion; epithelial-mesenchymal transition