南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (10): 1771-1775.doi: 10.12122/j.issn.1673-4254.2023.10.15

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丙泊酚诱导新生大鼠少突胶质细胞的凋亡：基于激活caspase家族蛋白和抑制抗凋亡程序

纪雪霞，朱 毅，张登文，蔡宇晶，周国斌   

  1. 南方医科大学附属广东省人民医院（广东省医学科学院）麻醉科，广东 广州 510080
  • 出版日期:2023-10-20 发布日期:2023-11-02

Mechanism of propofol-induced apoptosis in neonatal rat oligodendrocytes

JI Xuexia, ZHU Yi, ZHANG Dengwen, CAI Yujing, ZHOU Guobin   

  1. Department of Anesthesiology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China
  • Online:2023-10-20 Published:2023-11-02

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摘要： 目的 探讨丙泊酚诱导少突胶质细胞凋亡的分子机制。方法 采用随机数字表法将45只7 d SD乳鼠分为对照组（n=15）、脂肪乳剂组（n=15）和50 mg/kg丙泊酚组（n=15）。单次腹腔注射丙泊酚，0.4 mL/只（体积不足者用生理盐水补齐）。对照组和脂肪乳剂组腹腔注射等体积的生理盐水和中长链脂肪乳。丙泊酚腹腔注射麻醉8 h后，采用qPCR和Western blot检测不同组别caspase-3、caspase-8、caspase-9 mRNA和蛋白表达水平；检测NGF mRNA和P-PI3K/P-PAkt表达水平。结果 与对照组相比，丙泊酚组 caspase-3、caspase-8、caspase-9 mRNA 和蛋白表达水平升高（P<0.05）；NGF mRNA 和 P-PI3K/P-PAkt 表达水平降低（P<0.05）。结论 丙泊酚通过激活凋亡程序中caspase家族蛋白成员和抑制抗凋亡程序，诱导少突胶质细胞的凋亡。

关键词: 丙泊酚；少突胶质细胞；caspase家族；抗凋亡程序

Abstract: Objective To explore the molecular mechanism of propofol-induced apoptosis in oligodendrocytes. Methods Forty-five neonatal (7 days old) SD rats were randomized into 3 groups (n=15) for a single intraperitoneal injection of saline (control), long chain fat emulsion, or propofol (50 mg/kg). Eight hours after the injection, the rats were examined for mRNA and protein expressions of caspase-3, caspase-8, and caspase-9 in the brain tissues using qPCR and Western blotting, and the expression levels of nerve growth factor (NGF) mRNA and P-PI3K/P-PAkt were also detected. Results Compared with those in the control group, the neonatal rats with propofol injection showed significantly up-regulated mRNA and protein expressions of caspase-3, caspase-8, and caspase-9 in the brain tissue (P<0.05) with significantly down-regulated expressions of NGF mRNA and P-PI3K/P-PAkt (P<0.05). Conclusion Propofol induces apoptosis in oligodendrocytes by activating the caspase family protein members involved in triggering cell apoptosis and inhibiting the anti-apoptosis mechanism.

Key words: propofol; oligodendrocytes; caspase family protein; anti-apoptosis mechanism