南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (8): 1356-1362.doi: 10.12122/j.issn.1673-4254.2023.08.12

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姜黄素通过Keap1-Nrf2通路抑制甲状腺乳头状癌B-CPAP细胞的增殖、迁移及侵袭

任 丽，邹明远，朱行春，徐文隽，刘 刚，孙俊杰，范方田，张从利   

  1. 蚌埠医学院检验医学院，临床医学院，癌症转化医学安徽省重点实验室，药学院//安徽省生化药物工程技术研究中心，安徽 蚌埠 233030；第一附属医院麻醉科，安徽 蚌埠 233004
  • 出版日期:2023-08-20 发布日期:2023-09-13

Curcumin suppresses proliferation, migration and invasion of papillary thyriod cancer B-CPAP cells through the Keap1-Nrf2 pathway

REN Li, ZOU Mingyuan, ZHU Xingchun, XU Wenjun, LIU Gang, SUN Junjie, FAN Fangtian, ZHANG Congli   

  1. School of Laboratory Medicine, School of Clinical Medicine, Anhui Provincial Key Laboratory of Cancer Translational Medicine, School of Pharmacy/Anhui Biochemical Pharmaceutical Engineering Technology Research Center, Bengbu Medical College, Bengbu 233030, China; Department of Anesthesia, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China
  • Online:2023-08-20 Published:2023-09-13

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摘要： 目的 研究姜黄素对B-CPAP人甲状腺乳头状癌细胞增殖、迁移及侵袭的影响作用及其机制。方法 姜黄素（0、5、10、15、20 μmol/L）作用B-CPAP细胞，MTT法、Transwell法检测细胞存活率、迁移及侵袭；DCFH-DA试剂盒流式细胞仪检测细胞ROS水平；Western blot法验证Nrf2、Keap1蛋白变化情况，qRT-PCR法检测Nrf2、Keap1基因mRNA。结果 与0 μmol/L组细胞比较，随着姜黄素作用的时间（24、48、72 h）增加，B-CPAP细胞存活率显著下降，随着姜黄素浓度的增加细胞存活率、迁移、侵袭能力显著下降（P<0.05或P<0.01）；姜黄素剂量依赖性触发B-CPAP细胞ROS产生、并且可被N-乙酰半胱氨酸（NAC）有效逆转；20 μmol/L姜黄素组细胞Nrf2蛋白及mRNA水平显著降低、Keap1蛋白及mRNA水平表达显著升高（P<0.05或P<0.01），对细胞核、胞浆Nrf2蛋白水平的Western blot检测分析发现20 μmol/L姜黄素显著降低细胞核内Nrf2蛋白水平（P<0.05）、而胞浆内Nrf2蛋白表达量未见显著差异（P>0.05）。结论 姜黄素可能通过调节Keap1-Nrf2通路抑制B-CPAP甲状腺乳头状癌细胞增殖、迁移及侵袭能力。

关键词: 姜黄素；B-CPAP细胞；Keap1-Nrf2通路；甲状腺乳头状癌

Abstract: Objective To observe the effects of curcumin on migration and invasion of papillary thyriod cancer B-CPAP cells. Methods B-CPAP cells were treated with 5, 10, 15, or 20 μmol/L curcumin, and the changes in cell survival, migration and invasion were examined using MTT assay and Transwell assay. ROS levels in the treated cells were detected with a DCFH-DA probe. The expression levels of Nrf2 and Keap1 in the cells were determined using Western blotting and qRT-PCR. Results Treatment with curcumin dose- and time-dependently suppressed the viability of B-CPAP cells (P<0.05 or P<0.01). Curcumin inhibited the migration and invasion (P<0.001) and promoted ROS production in B-CPAP cells in a dose-dependent manner, and application of NAC effectively reversed curcumin- induced increase of ROS. Curcumin at 20 μmol/L significantly decreased the protein and mRNA expressions of Nrf2 and increased the expressions of Keap1 protein and mRNA (P<0.05 or P<0.01), causing also significantly reduced expression of Nrf2 protein in the cell nuclei (P<0.05) without obviously affecting its expression in the cytoplasm (P>0.05). Conclusion Curcumin inhibits the proliferation, migration and invasion of papillary thyriod cancer B-CPAP cells probably via the Keap1-Nrf2 signaling pathway.

Key words: curcumin; B-CPAP; Keap1-Nrf2 pathway; papillary thyriod cancer