南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (7): 1172-1178.doi: 10.12122/j.issn.1673-4254.2023.07.14

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大鼠颈上神经节代谢型谷氨酸受体7，8的表达及慢性间歇性低氧对其的影响

魏茜茜，李超红，赵晨露，赵宝生，刘玉珍   

  1. 新乡医学院第一附属医院河南省神经修复重点实验室，生命科学研究中心，胸外科，河南 卫辉 453100
  • 出版日期:2023-07-20 发布日期:2023-07-20

Characterization of metabotropic glutamate receptor 7 and 8 in rat superior cervical ganglion and their changes following chronic intermittent hypoxia

WEI Xixi, LI Chaohong, ZHAO Chenlu, ZHAO Baosheng, LIU Yuzhen   

  1. Henan Key Laboratory of Neural Regeneration, Life Science Research Center, Department of Theoretic Surgery, First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China
  • Online:2023-07-20 Published:2023-07-20

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摘要： 目的 研究大鼠颈上神经节（SCG）组织中III组代谢型谷氨酸受体7和8（mGluR7/8）的表达、定位，以及慢性间歇性低氧（CIH）对mGluR7和mGluR8蛋白表达量的影响。方法 选取8周龄雄性SD大鼠30只，免疫组织化学染色检测大鼠SCG组织中mGluR7 和 mGluR8 的表达。免疫荧光共定位染色检测 mGluR7 和 mGluR8 的分布。核浆分离蛋白进行 Western blot检测mGluR7和mGluR8在细胞浆和细胞核的分布情况。构建6周CIH大鼠模型，CIH组放于低氧小室中：首先向小室内充入100%氮气，使氧浓度分数逐渐降低至6% 并维持30 s，然后充入100% 氧气，使氧浓度分数回升至21%，作用周期为4 min/次，8 h/d，对照组放于常氧环境中。通过尾动脉血压检测大鼠收缩压、舒张压和平均动脉压的变化。Western blot检测CIH对SCG中mGluR7和mGluR8蛋白表达量的影响。结果 大鼠SCG表达mGluR7和mGluR8。mGluR7分布于神经元和小荧光（SIF）细胞，上述细胞胞浆和胞核中均染色阳性，而在卫星胶质细胞（SGCs）、神经纤维和血管中不表达；mGluR8定位于神经元和SIF细胞的胞浆中，而在SGCs、神经纤维和血管中不表达。大鼠SCG核浆蛋白检测结果进一步证实mGluR7不仅存在于胞浆也存在于胞核蛋白中，而mGluR8仅存在于胞浆蛋白中。动物实验结果显示，与对照组相比，CIH升高大鼠的收缩压（P<0.0001）、舒张压（P<0.001）和平均动脉压（P<0.0001）。CIH增加mGluR7和mGluR8的蛋白表达量（P<0.05）。结论 mGluR7和mGluR8均表达于大鼠SCG，但是细胞定位方式不同，CIH升高大鼠血压并增加mGluR7和mGluR8蛋白在SCG的表达水平。

关键词: 代谢型谷氨酸受体7；代谢型谷氨酸受体8；颈上神经节；慢性间歇性低氧；高血压

Abstract: Objective To investigate the expression and localization of metabotropic glutamate receptors 7 and 8 (mGluR7/8) in rat superior cervical ganglion (SCG) and their changes in response to chronic intermittent hypoxia (CIH). Methods We detected the expressions of mGluR7 and mGluR8 in the SCG of 8-week-old male SD rats using immunohistochemistry and characterized their distribution with immunofluorescence staining. The expression of mGluR7 and mGluR8 in the cytoplasm and nucleus was detected using Western blotting. A 6-week CIH rat model was established by exposure to intermittent hypoxia (6% oxygen for 30 s followed by normoxia for 4 min) for 8 h daily, and the changes in systolic blood pressure, diastolic blood pressure and mean arterial pressure were measured. The effect of CIH on expression levels of mGluR7 and mGluR8 in the SCG was analyzed using Western blotting. Results Positive expressions of mGluR7 and mGluR8 were detected in rat SCG. mGluR7 was distributed in the neurons and small fluorescent (SIF) cells with positive staining in both the cytoplasm and nuclei, but not expressed in satellite glial cells (SGCs), nerve fibers or blood vessels; mGluR8 was localized in the cytoplasm of neurons and SIF cells, but not expressed in SGCs, nerve fibers, or blood vessels. Western blotting of the nuclear and cytoplasmic fractions of rat SCG further confirmed that mGluR7 was expressed in both the cytoplasm and the nucleus, while mGluR8 exists only in the cytoplasm. Exposure to CIH significantly increased systolic blood pressure, diastolic blood pressure and mean arterial pressure of the rats (all P<0.001) and augmented the protein expressions of mGluR7 and mGluR8 in the SCG (P<0.05). Conclusion mGluR7 and mGluR8 are present in rat SCG but with different localization patterns. CIH increases blood pressure of rats and enhanced protein expressions of mGluR7 and mGluR8 in rat SCG.

Key words: metabotropicglutamatereceptors 7; metabotropicglutamatereceptors 8; superior cervical ganglion; chronic intermittent hypoxia; hypertension