南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (3): 368-374.doi: 10.12122/j.issn.1673-4254.2023.03.05

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CD5L在念珠菌血症患者血清中的水平升高并加重模型小鼠的病情

吴夏楠，杨 铭，郭文涛，呼 洁，董 轲，郜赵伟   

  1. 空军军医大学第二附属医院检验科，陕西 西安 710038
  • 出版日期:2023-03-20 发布日期:2023-03-20

CD5L is elevated in the serum of patients with candidemia and promotes disease progression in mouse models

WU Xianan, YANG Ming, GUO Wentao, HU Jie, DONG Ke, GAO Zhaowei   

  1. Clinical Laboratory, Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China
  • Online:2023-03-20 Published:2023-03-20

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摘要： 目的 检测CD5L水平念珠菌血症患者中的变化，并研究CD5L对念珠菌血症疾病发展的作用。方法 选取健康对照者20例，细菌血症患者27例和念珠菌血症患者35例，ELISA检测各组血清中的CD5L水平。分析CD5L水平与患者血清中感染及肾损伤指标之间的相关性。尾静脉注射白色念珠菌至C57BL/6小鼠，构建念珠菌血症小鼠模型。腹腔注射CD5L蛋白，利用组织病理及血清学检测分析小鼠模型肾损伤，检测肾脏菌载量，统计小鼠生存率，分析CD5L处理对念珠菌血症小鼠病情的影响。ELISA检测CD5L处理对念珠菌血症小鼠血清IL-6及IL-8水平的影响。结果 与健康对照及细菌感染患者相比，念珠菌血症患者血清CD5L水平显著升高（P<0.01），且 CD5L水平与白细胞数（WBC）、1, 3-β-D-葡聚糖（BDG）、肌酐（Scr）和降钙素原（PCT）水平呈正相关。与正常小鼠相比，念珠菌血症小鼠血清及肾脏CD5L水平显著升高（P<0.01）。腹腔注射CD5L蛋白可以显著上调念珠菌血症小鼠肾组织菌载量，促进小鼠表达炎症因子IL-6和IL-8，加重小鼠肾脏损伤，降低小鼠生存率（P<0.05）。结论 血清CD5L水平在念珠菌血症患者中升高，CD5L加重念珠菌血症小鼠病情。

关键词: 念珠菌血症；CD5L；炎症；肾损伤

Abstract: Objective To investigate the changes of CD5L levels in patients with candidemia and explore the role of CD5L in progression of candidemia. Methods Twenty healthy control individuals, 27 patients with bacteremia and 35 patients with candidemia were examined for serum CD5L levels using ELISA, and the correlations of CD5L level with other serological indicators were analyzed. A C57BL/6 mouse model of candidemia induced by intravenous injection of Candida albicans were treated with intraperitoneal injection of recombinant CD5L protein, and renal histopathological and serological changes were analyzed to assess renal injures. The effects of CD5L treatment on general condition, fungal burden, of survival of the mice were observed, and the changes in serum IL-6 and IL-8 levels of the mice were detected using ELISA. Results CD5L levels were significantly elevated in patients with candidemia and positively correlated with WBC, BDG, Scr and PCT levels. The mouse model of candidemia also showed significantly increased serum and renal CD5L levels, and CD5L treatment significantly increased fungal burden in the renal tissue, elevated IL-6 and IL-8 levels in the serum and kidney, aggravated renal tissue damage, and reduced survival rate of candidemia mice. Conclusion Serum CD5L levels are increased in patients with candidemia, and treatment with CD5L aggravates candidemia in mouse models.

Key words: candidiasis; CD5L; inflammation; renal injury