南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (1): 117-121.doi: 10.12122/j.issn.1673-4254.2023.01.16

• • 上一篇    下一篇

白兔局部动脉粥样硬化不稳定斑块对其余血管斑块形成的影响

陆 琦，鲍迎春，陈旭姣，王春明   

  1. 宁波大学附属人民医院心内科，浙江 宁波 315040；宁波市医疗中心李惠利医院东部院区特检科，浙江 宁波 315048
  • 出版日期:2023-01-20 发布日期:2023-02-22

Effect of local unstable atherosclerotic plaque on plaque formation in the carotid artery and abdominal aorta of rabbits

LU Qi, BAO Yingchun, CHEN Xujiao, WANG Chunming   

  1. Department of Cardiology, Affiliated People's Hospital of Ningbo University, Ningbo 315040, China; Eastern Brach of Lihuili Hospital, Ningbo Medical Center, Ningbo 315048, China
  • Online:2023-01-20 Published:2023-02-22

RichHTML

17

PDF

169

摘要： 目的 分析局部动脉粥样硬化不稳定斑块对其余动脉粥样硬化斑块形成的影响。方法 选取体质量年龄适合的新西兰白兔30只，按照随机数字表法将其随机分为3组（n=10）。实验组和对照组予以高脂饲料喂养，空白组予以普通饲料喂养。喂养4周后，对实验组采用颈动脉球囊损伤术进行动脉粥样硬化建模，12周后杀死兔子，取颈动脉、腹主动脉进行病理组织学检测，利用图像分析软件测量各部位平均内膜厚度、斑块面积与管腔面积之比，同时抽取兔子静脉血行血脂分析。结果 4周末、8周末、12周末时，实验组、对照组体质量较0周时明显升高（P<0.05），空白组体质量较0周时无明显差异（P>0.05），且空白组体质量明显低于实验组、对照组（P<0.05），对照组间体质量比较无明显差异（P>0.05）；实验组平均内膜厚度明显大于对照组、空白组（P<0.05），且实验组手术侧颈动脉平均内膜厚度、斑块面积与管腔面积明显大于对侧颈动脉、腹主动脉（P<0.05）；12周末时，实验组、对照组甘油三酯（TG）、总胆固醇（TC）、低密度胆固醇（LDL-C）、高密度胆固醇（HDL-C）、超敏C反应蛋白（CRP）水平明显高于空白组（P<0.05），且对照组间TG、TC、LDL-C、HDL-C、CRP水平比较无明显差异（P>0.05），实验组CRP水平明显高于对照组（P<0.05）。结论 局部动脉粥样硬化不稳定斑块可使机体CRP水平升高，进而促进其余动脉粥样硬化斑块的形成。

关键词: 动脉粥样硬化；不稳定斑块；外周血管

Abstract: Objective To analyze the effect of local unstable atherosclerotic plaque on plaque formation in the carotid and aortic arteries of rabbits. Methods Thirty New Zealand white rabbits were randomly divided into atherosclerosis model group, high-fat diet feeding group, and normal chow feeding group (blank control group). In the model group, carotid artery balloon injury was induced after 4 weeks of high-fat diet feeding. Eight weeks later, all the rabbits were euthanized for histopathological examination of the carotid artery and abdominal aorta, and the mean intimal thickness and plaque to lumen area ratio were measured using image analysis software. Venous blood samples were collected from the rabbits for blood lipid analysis. Result At the ends of 4, 8 and 12 weeks, the rabbits in the model group and high-fat feeding group, but not those in the blank control group, all showed significant weight gain compared with their body weight at 0 week (P<0.05). The mean intimal thickness was significantly greater in atherosclerosis model group than in the other two groups (P<0.05). In atherosclerosis model group, the mean intimal thickness and plaque to lumen area ratio in the injured carotid artery were significantly greater than those in the contralateral carotid artery and abdominal aorta (P<0.05). At the end of the 12 weeks, the levels of triglyceride (TG), total cholesterol (TC), low-density cholesterol (LDL-C), high-density cholesterol (HDL-C) and high-sensitivity C- reactive protein (CRP) were all significantly higher in the model group and high- fat feeding group than in the blank control group (P<0.05); the levels of TG, TC, LDL-C, or HDL-C did not differ significantly between the model group and high- fat feeding group (P>0.05), but the level of CRP was significantly higher in arteriosclerosis model group (P<0.05). Conclusion Local unstable atherosclerotic plaque can increase the level of CRP and promote the formation of atherosclerotic plaques in the carotid artery and abdominal aorta in rabbits.

Key words: atherosclerosis; unstable plaques; peripheral arteries