南方医科大学学报

南方医科大学学报 ›› 2022, Vol. 42 ›› Issue (12): 1889-1895.doi: 10.12122/j.issn.1673-4254.2022.12.19

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小鼠纹状体尾部和听皮层PV神经元的电生理性质比较

游佳鹏，宋长宝，梁妃学   

  1. 南方医科大学生物医学工程学院神经信息工程实验室，广东 广州 510515
  • 出版日期:2022-12-20 发布日期:2023-01-12

Comparison of electrophysiological properties of parvalbumin neurons in the tail of the striatum and the auditory cortex of mice

YOU Jiapeng, SONG Changbao, LIANG Feixue   

  1. School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China
  • Online:2022-12-20 Published:2023-01-12

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摘要： 目的 研究小白蛋白（PV）神经元在听皮层（AC）及其下行听觉投射脑区纹状体尾部（TS）的电生理性质异同。方法 以PV-Cre-Ai14小鼠为研究对象，运用离体膜片钳技术记录PV神经元对步进电流的刺激响应，并通过Clampfit和MATLAB软件分析PV神经元动作电位（AP）的发放特性和波形特性。AP的发放特性包括延时、阈值、平均放电率、F/I斜率以及动作电位频率自适应性(SFA)；波形特性包括峰电位特性和后电位特性。实验结束后统计共使用PV-Cre-Ai14小鼠5只，共记录到29个TS-PV神经元和22个AC-PV神经元。结果 TS和AC的PV神经元存在显著不同的电生理特性。从峰电位特性上看，TS的PV神经元具有更小的半峰宽（P<0.001）和更大的幅值（P<0.01），AP的最大上升斜率（P<0.01）和最大下降斜率（P<0.05）也更大；从后电位特性上看，TS的PV神经元后超极化程度更大（P<0.01），恢复到静息电位的时间更短（P<0.01）；从发放特性上看，TS的PV神经元有更高的AP发放阈值（P<0.01）、更大的F/I斜率（P<0.01）和发放延时（P<0.001），其SFA也更大（P<0.01）。结论 TS中的PV神经元和AC中的PV神经元在处理听觉信息的过程中表现出显著不同的电生理特性。

关键词: 纹状体尾部；听皮层；小白蛋白神经元；电生理性质

Abstract: Objective To study the electrophysiological properties of parvalbumin (PV) neurons in the auditory cortex (AC) and its descending auditory projection area in the tail of the striatum (TS). Methods The stimulation response of PV neuron step current was recorded in PV-Cre-Ai14 mice using in vitro patch clamp technique, and the release characteristics and waveform characteristics of PV neuron action potentials (APs) were analyzed using Clampfit and MATLAB software. The release characteristics of the APs included AP onset, rheobase, average firing rate, F/I slope and spike frequency adaptation (SFA); the waveform characteristics included peak and post potential characteristics. Results The PV neurons of the TS and the AC had significantly different electrophysiological characteristics. In terms of peak potential characteristics, the PV neurons in the TS presented with smaller half peak width (P<0.001) and larger amplitude (P<0.01) with larger maximum ascending slope (P<0.01) and maximum descending slope (P<0.05). For post potential characteristics, the PV neurons in the TS showed a greater post hyperpolarization (P<0.01) with a shorter time for recovery of the resting potential (P<0.01). The firing characteristics of the PV neurons of the TS featured a higher AP rheobase (P<0.01), a larger F/I slope (P<0.01), a greater firing onset delay (P<0.001), and a larger SFA (P<0.01). Conclusion The PV neurons in the TS and the AC of mice show significantly different electrophysiological characteristics in processing auditory information.

Key words: the tail of the striatum; auditory cortex; parvalbumin neuron; electrophysiological properties