南方医科大学学报

南方医科大学学报 ›› 2022, Vol. 42 ›› Issue (11): 1732-1738.doi: 10.12122/j.issn.1673-4254.2022.11.19

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基于氧化亚铜纳米颗粒的光热/化学动力协同策略体外抑制胃癌细胞的迁移

温海飞，黄显莹   

  1. 南方医科大学南方医院第一临床医学院，广东 广州 510515
  • 出版日期:2022-11-20 发布日期:2022-11-30

Cuprous oxide nanoparticles-based photothermal and chemodynamic synergistic therapy inhibits proliferation and migration of gastric cancer cells in vitro

WEN Haifei, HUANG Xianying   

  1. Nanfang Hospital, First Clinical Medical College, Southern Medical University, Guangzhou 510515, China
  • Online:2022-11-20 Published:2022-11-30

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摘要： 目的 探讨氧化亚铜（Cu2O）纳米的制备、物化表征及其胃癌抗肿瘤效应的作用。方法 采用透射电镜，动态光散射，Zeta电位分析及紫外吸收光谱分析方法分析Cu2O纳米的形貌、粒径大小及类芬顿性能，利用不同浓度的Cu2O纳米对细胞进行处理，并进行光照或者非光照处理，并分别采用细胞增殖-毒性实验（CCK-8）、Transwell细胞迁移实验评估该纳米的体外抗肿瘤作用效应。结果 制备的Cu2O纳米为100 nm左右的类圆形结构，在近红外光（NIR, 0.5/cm2）照射5 min后Cu2O纳米温度从25 ℃提高到50 ℃，同时Cu2O纳米在过氧化氢浓度和pH为近中性的条件下（pH=6.5）可催化大量活性氧（ROS）生成。CCK-8试验表明：相比于对照组，Cu2O纳米对胃癌细胞具有浓度依赖性抑制增殖作用；Transwell细胞迁移实验表明：Cu2O纳米能抑制胃癌细胞的迁移能力。结论 Cu2O铜基纳米颗粒具备良好的光热性能、化学动力性能及抗肿瘤效应，是胃癌治疗的一种潜在新方法。

关键词: 氧化亚铜；纳米；化学动力疗法；光热疗法

Abstract: Objective To investigate the physicochemical characterization of cuprous oxide (Cu2O) nanoparticles and assess its antitumor effect against gastric cancer cells in vitro. Methods The morphology, particle size and Fenton-like properties of Cu2O nanoparticles were analyzed using transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential analysis and ultraviolet absorption spectroscopy. CCK-8 assay and Transwell experiments were used for evaluating the in vitro anti-tumor effect of the nanometers in gastric cancer cells. Results The prepared Cu2O nanoparticles had a quasi-circular structure with a diameter of about 100 nm. The temperature of the nanoparticles increased from 25 to 50 ℃ after irradiation with near-infrared light (NIR, 0.5W/cm2) for 5 min. At a nearly neutral pH (pH=6.5), the nanoparticles catalyzed the generation of a large amount of reactive oxygen species (ROS). CCK-8 assay and Transwell experiment showed that Cu2O nanoparticles concentration-dependently inhibited the proliferation, invasion and migration of gastric cancer cells. Conclusion Cu2O nanoparticles have good photothermal and chemokinetic properties with a strong anti-tumor effect, and can potentially serve as a new therapeutic agent for gastric cancer treatment.

Key words: cuprous oxide; nanoparticles; chemodynamic therapy; photothermal therapy