南方医科大学学报

南方医科大学学报 ›› 2021, Vol. 41 ›› Issue (7): 1101-1106.doi: 10.12122/j.issn.1673-4254.2021.07.20

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2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷可减轻脂多糖诱导的大鼠急性肺损伤

陈加宝，刘 华
  

  • 出版日期:2021-07-20 发布日期:2021-07-16

2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-d-glucoside alleviates lipopolysaccharide-induced acute lung injury in rats

  • Online:2021-07-20 Published:2021-07-16

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摘要： 目的 观察2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG）对脂多糖（LPS）诱导的大鼠急性肺损伤（ALI）的保护作用，探讨其相关机制。方法 将36只SD大鼠随机分为正常对照组（n=9，蒸馏水 10 mL/kg，灌胃）、模型组（LPS组，n=9，脂多糖5 mg/kg，尾静脉注射）、TSG低剂量组（n=9, TSG 50 mg/kg，灌胃）和TSG高剂量组（n=9, TSG 100 mg/kg，灌胃）6 h后处死大鼠，观察大鼠肺组织病理切片并行肺损伤评分，测定肺组织湿/干质量比（W/D），ELISA法检测血清中炎症因子TNF-α和IL-6的浓度，同时检测肺组织中超氧化物歧化酶（SOD）的活力和丙二醛（MDA）的含量，并运用Western blot检测肺组织中NF-κB p65的表达水平。结果 与LPS组相比，TSG低、高剂量组肺病理损伤明显减轻（P<0.001），血清TNF-α和IL-6的浓度显著降低（P<0.001），肺组织W/D明显下降（P<0.001），SOD活性明显升高（P<0.001），MDA含量明显减少（P<0.001），NF-κB p65的表达水平显著下降，差异均具有统计学意义（P<0.001）。而TSG低剂量组和高剂量组之间比较并无差异（P 病理损伤评分=0.99，PTNF-α=0.99，PIL-6=0.86，PW/D=0.84， PSOD=0.76，PMDA=0.74，PNF-κB p65=0.17）。结论 TSG可减轻脂多糖诱导的急性肺损伤，其保护作用可能与抑制NF-κB通路的激活，提高抗氧化能力，减少炎症因子的释放有关。

关键词: 2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷；脂多糖；急性肺损伤；NF-κB

Abstract: Objective To observe the protective effect of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-d-glucoside (TSG) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and explore the underlying mechanism. Methods Thirty-six SD rats were randomized equally into 4 groups: the normal control group, ALI model group, and low- and high-dose TSG groups (treated with 50 and 100 mg/kg TSG via intragastric administration, respectively). In all but the normal control group, the rats were subjected to tail vein injection of LPS to induced ALI. The rats were euthanized at 6 h after the injection for pathological examination of the lungs. The wet/dry weight ratio (W/D) of the lungs were calculated, and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the lung tissues and serum levels of TNF-α and IL-6 were determined. Western blotting was performed to detect the levels of NF-κB p65 in the lungs. Results Compared with those in LPS group, the TSG-treated rats showed significantly milder lung pathologies (P<0.001) and had lower serum TNF-α and IL-6 levels (P<0.001) and W/D of the lung tissues (P<0.001), higher SOD activity (P<0.001) and lower MDA content in the lungs (P<0.001), and significantly lower expression of NF-κB p65 in the lungs (P<0.001). None of these indices showed significant differences between the low and high- dose TSG treatment groups (P>0.05). Conclusions TSG can ameliorate LPS- induced ALI in rats possibly by suppressing the NF-κB pathway to improve the antioxidant capacity and decrease the release of inflammatory factors.

Key words: 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-d-glucoside; lipopolysaccharide; acute lung injury; nuclear factor κB